摘要
目的用小鼠巨细胞病毒(murine cytomegalovirus, MCMV)K181株建立BALB/c小鼠巨细胞病毒性心肌炎模型.方法 25只健康纯系5周龄BALB/c小鼠腹腔接种MCMV K181病毒悬液(1×104PFU/只),观察小鼠血清cTnI浓度变化和心肌组织MCMV病毒滴度及病理改变.结果小鼠腹腔接种病毒悬液后第3天即可在心肌组织中检测到低滴度MCMV,第5～7天达高峰,随后迅速下降,第10天时已不能检测到MCMV;小鼠心肌组织病理改变出现在MCMV感染后第3天,7～10天达高峰,随后逐渐减轻,病变可持续到病毒感染后3～4个月;血清cTnI水平在MCMV感染后第3天即有升高,7～10天达高峰,14天仍维持较高水平.结论成功建立小鼠巨细胞病毒性心肌炎模型,可为巨细胞病毒性心肌炎的治疗、药物筛选、疾病发病机制以及疾病预后评估提供一个良好研究平台.
Objective To establish a BALB/c mice model system of cytomegalovirus-induced myocarditis. Methods Twenty five specific pathogen-free inbred female BALB/c mice (5 weeks old, 16-18 g, seronegative for MCMV) were infected with 1×104 PFU MCMV by the intraperitoneal (i.p.) route. All experimental mice were sacrificed at 3, 5, 7, 10,14 days i. p. (n=5 per time point). Hearts were removed under aseptic conditions, and were transected along the midline. One part of each heart was processed with Bouin′s fixative for histological examination. The other part of each heart was immediately frozen in liquid nitrogen and stored at -80℃ until MCMV titre was determined by plaque assay. Seurm cTnI level was assayed by ELISA. Results MCMV was detected in the hearts at exaremely low levels on 3 days i. p. and could not be detected on 10 days i. p. A mixed cellular infiltrate composed of polymorphonuclear neutrophils and mononuclear lymphocytes was observed on 3 days, which reached a peak at 7 to 10 days after MCMV infection and was maintained for at least 3-4 months postinfection. Seurm cTnI levels were elevated on 3 days i. p., reaching a peak at 7 to 10 days i. p..Conclusions These data highlight the possible therapeutic uses of antiviral drugs in viral myocarditis as well as further elucidating the pathogenic nature of the disease.
出处
《中华心血管病杂志》
CAS
CSCD
北大核心
2005年第4期360-363,共4页
Chinese Journal of Cardiology
