摘要
目的应用抗人cTnI单克隆抗体检测大鼠血清cTnI。方法SD大鼠皮下注射异丙肾上腺素8mg/kg,对照组大鼠注射等体积的生理盐水,4h后处死,观察心肌组织病理改变;应用2株抗人cTnI单克隆抗体建立双抗体夹心ELISA法检测大鼠血清cTnI。结果大鼠注射异丙肾上腺素后心肌呈散在局灶性病变,表现为心肌纤维呈波浪样改变,部分心肌纤维断裂,心肌间质内炎细胞浸润、水肿。血清cTnI(OD值)明显高于对照组大鼠(1.28±0.78∶0.30±0.22,P<0.01)。结论本实验建立了大鼠血清cTnI双抗体夹心ELISA方法。血清cTnI能反映大鼠心肌病理变化,可作为诊断大鼠心肌损伤的一个可靠指标。
Objective: To detect serum cTnI of rats. Methods: Myocardial injury of the rat was induced by isoprenaline. The serum cTnI of the rats was determined by sandwich enzyme immunoassay in which two cTnI-specific murine monoclonal antibodies were used. Results: The histological examinations of the myocardium showed alterations of limited local degeneration of muscle fibres, interstitial oedema and mild neutrophilic infiltration. The serum cTnI of isoprenaline-treated rats was significantly higher than that of the control rats. Conclusion: The present method could detect changes of serum cTnI of the rat.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2004年第5期477-479,共3页
Journal of Nanjing Medical University(Natural Sciences)
基金
江苏省135临床生物学诊断与治疗重点实验室基金资助项目(SKZ200205)
江苏省科技厅科研基金资助项目(BS2002045)
江苏省高新技术研究项目(BG2003033)
