摘要
目的：建立由柯萨奇病毒B3（CVB3）Nancy株诱导的病毒性心肌炎（VMC）C57BL/6J小鼠模型。方法：4周龄、6周龄C57BL/6J小鼠各50只,每个年龄组随机选取15只作为对照（腹腔注射PBS）,剩余35只腹腔接种含CVB3 Nancy株的病毒悬液（1×10^5PFU/只）,于感染后第14天取心脏,行HE染色和CD3、CD45免疫组化染色,进行病理学评价,检测血清白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）水平,并做心肌组织中病毒滴度检测,采用Western blot法检测磷酸化AKT、Ik Bα、GSK3β的表达。结果：4周龄小鼠造模过程中有19只（54.3%）死亡;与对照小鼠比较,模型小鼠心肌呈现弥漫性炎性细胞浸润和片状心肌细胞变性坏死,CD3、CD45阳性细胞弥漫浸润于心肌间质,血清IL-1β、TNF-α水平明显升高（P〈0.05）,心肌组织中磷酸化AKT、Ik Bα、GSK3β表达增高（P〈0.05）。6周龄小鼠造模过程中有3只（8.6%）死亡,模型小鼠心肌组织中炎症反应轻于4周龄模型小鼠（P〈0.05）。结论：成功建立了VMC的C57BL/6J小鼠模型,4周龄C57BL/6J小鼠更适用于模型的建立。
Aim：To establish a C57BL/6J mouse model of myocarditis induced by coxsackievirus B 3（CVB3）.Meth-ods：A total of 50 male mice with 4-week-age or 6-week-age were divided into two groups：the control（n=15） were injec-ted via intraperiton with PBS, and the model group（n=35） were injected via intraperiton with 1×10^5 PFU CVB3.After 14 d, mice hearts were harvested for HE staining and CD 3,CD45 immunohistochemistry staining ,the serum levels of IL-1β,TNF-αwere assayed by ELISA , the expressions of p-AKT,p-IkBαand p-GSK3βwere detected by Western blot .Re-sults：4-week-age model group came out with a higher mortality （19/35,54.3%）, HE staining showed that a great deal of inflammatory cells concentrated in the adjacent area around the myocardiocytes ,which was consistent with the finding that many CD3,CD45 positive macrophages gathered in the necrosis area ,TNF-αand IL-6 level were increased（P〈0.05）,p-AKT,p-IκBαand p-GSK3βprotein expression increased （P〈0.05）.While the 6-week-age model group showed a lower mortality（3/35,8.6%）,and the immune response to CVB3 infection was milder than 4-week-age model group（P〈0.05）. Conclusion：A C57BL/6J mouse model of myocarditis induced by CVB 3 is successfully established ,and 4-week-age mice are recommended for this model .
出处
《郑州大学学报（医学版）》
CAS
北大核心
2016年第2期212-217,共6页
Journal of Zhengzhou University(Medical Sciences)
作者简介
通信作者，女，1956年4月生，硕士，主任医师，研究方向：心血管内科疾病，E—mail：zhaoluosha@126．com
