摘要
目的研究免疫性血小板减少症（ITP）患者树突细胞（Dc）亚群和淋巴转录因子Gata-3和T-het表达异常及其相关性。方法选取2012年2至11月天津医科大学总医院血液科16例初治ITP患者、17例恢复期ITP患者，采用流式细胞术检测患者外周血浆细胞样树突细胞（pDC）（LinHLA—DRCD123＋）和髓样树突细胞（mDC）（LinHLA—DR＋）数值及比例，RT—PCR检测外周血Gata-3mRNA和T—betmRNA表达，流式细胞数检测外周血干扰素γ（IFN-γ）、白细胞介素4（IL4）水平。以12名健康体检者作为对照组。结果ITP仞治组pDC占外周血单个核细胞（PBMNC）比例为0．49％±0．18％，明显高于恢复组和健康对照组（0．27％±0．17％、0．32％±0．13％，均P〈0．05）；mDC占PBMNC比例为0．23％±0．17％，低于恢复组和埘照组（0．33％±0．18％、0．31％±0．11％），但差异无统计学意义（P〉0．05）。初治组pDC／mDC比例（3．15±2．01）高于恢复组（0．81±0．32）及对照绢（1．07±0．44）（均P〈0．05）。3组Gata-3mRNA表达量分别是（2775±489、1357±307、652±165）；仞治组明显高于恢复组和对照组（均P〈0．05）。初治组、恢复组及对照组T—betmRNA的表达量分别是782±394、583±176、576±120，3组间差异无统计学意义（P〉0．05）；初治组Gata-3／T—bet比例（4．13±1．69）明显高于恢复组（2．45±0．69）和对照组（1．15±0．27）（均P〈0．05）。初治组外周咀血2细胞因子IL-4明显高于恢复组和对照组（9．14％±4．34％比4．78％±1．69％、4．86％±1．41％，均P〈0．05）；初治组IFN-γ低于对照组（P〈0．05）。Gata-3mRNA表达水平与pDC／mDC比例和IL-4水平呈显著正相关（r=0．585、0．463，均P〈0．05）。结论ITP发病可能与DC亚群失衡及下游淋巴转录因子Gata-3表达增高相关。
Objective To explore the relationship between the dendritic cell （DC） subsets and abnormal expression of transcription factors Gata-3 and T-bet in patients with immune thrombocytopenia （1TP）. Methods The plasmacytoid DC （pDC） and myeloid DC （mDC） of 33 ITP （16 untreated, 17 remitted） patients and 12 healthy controls were analyzed by flow cytometry（FCM）. The expressions of Gata-3 mRNA and T-bet mRNA in peripheral blood mononuclear cell （PBMNC） were detected by reverse transcription-polymerase chain reaction （ RT-PCR ） . The levels of interleukin-4 （ IL-4 ） and interferon- gamma （IFN-γ） were measured by FCM in 33 ITP patients and 12 healthy controls. Results The percentage of pDC in PBMNC was 0. 49% ＋0. 18% in untreated and it was higher than that in remitted ITP patients （0. 27% ± 0. 17% ） and in controls （0. 32% ±0. 13% ） （both P 〈 0. 05 ）. The percentage of mDC in PBMNC was 0. 23% ±0. 17% in untreated,which was lower than that in remitted ITP patients （0. 33% ±0. 18）% and in controls（0. 31% ±0. 11% ） ,but no statistic; difference in mDC expression existed among 3 groups （P 〉0. 05）. pDC/mDC ratios was （3.15 ±2. 01 ） in untreated ITP patients and it was higher than that in remitted ITP patients （0. 81 ±0.32） and in controls （ 1.07 ±0.44） （ both P 〈0.05 ）. The relative mRNA expression levels of Gata-3 were 2775 ±489, 1357 ±307 and 652 ± 165 respectively. And the expression of Gata-3mRNA in untreated group was higher than that in remission group or healthy controls （ both P 〈 0. 05）. The relative mRNA expression levels of T-bet were 782 ±394, 583 ±176 and 576 ±120. No statistic difference in T-bet expression existed among 3 groups （P 〉 0. 05 ）. Gata-3mRNA/T-bet mRNA ratio was （4. 13 ±1.69 ） in untreated group and it was higher than that of remission group （2. 45 ±0. 69） or controls （ 1.15 ±0. 27 ） （ both P 〈 0. 05 ）. The level of IL-4 in the untreated group was 9.14% ±4. 34% and it was higher than that of remission group （4. 78% ±1.69% ） or controls （4. 86% ±1.41% ）. The level of IFN-γ in the untreated group was lower than that of controls （ P 〈 0.05 ）. Significant positive correlations existed between Gata-3 and pDC/mDC ratio （r = 0. 585, P 〈 0. 01 ）. Significant positive correlations existed between Gata-3 and IL-4 （ r = 0. 463, P 〈 0. 05 ）. Conclusion The mechanism of ITP may be due to a disorder of DC subsets and a high expression of Gata-3.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2013年第22期1696-1699,共4页
National Medical Journal of China
基金
国家自然科学基金，卫生部卫生行业科研专项，天津市卫生局科技攻关项目，天津市自然科学基金，中华医学会分子生物学临床应用研究专项基金
