摘要
目的:研究正常环境和高钙环境下,大鼠不同肠段中盐酸小檗胺对盐酸小檗碱肠吸收特性的影响。方法:采用大鼠外翻肠囊模型,通过HPLC检测不同配伍组外翻肠囊吸收液中盐酸小檗碱的含量,对不同配伍组的单位面积吸收量进行单因素方差分析。结果:与相同时间点正常J70组(正常环境,盐酸小檗碱质量浓度70 mg·L-1)比较,120 min时正常J70+Ver100组(正常环境,盐酸小檗碱质量浓度70 mg·L-1,加入盐酸维拉帕米质量浓度100 mg·L-1)的单位面积吸收量在回肠段显著增加(P<0.05);30,60,90 min时高钙J70+A35组(高钙环境,盐酸小檗碱质量浓度70 mg·L-1,加入盐酸小檗胺质量浓度35 mg·L-1)在十二指肠的单位面积吸收量显著增加(P<0.05);30,90,120 min时高钙J70+A70组(高钙环境,盐酸小檗碱质量浓度70 mg·L-1,加入盐酸小檗胺质量浓度70 mg·L-1)在回肠的单位面积吸收量显著增加(P<0.05)。相较于相同质量浓度的正常组,高钙J70+A35组、高钙J70+A70组中盐酸小檗碱的肠吸收更好。结论:盐酸小檗胺在一定程度上对盐酸小檗碱的吸收具有促进作用,尤其是在高钙环境下。
Objective:To investigate the effect of berbamine hydrochloride on the absorption characteristics of berberine hydrochloride in different intestinal segments of rats in normal environment and high calcium environment.Method:Taking rat everted intestinal sac model,the content of berberine hydrochloride in absorbent solution of everted intestinal sac from different compatibility groups was determined by HPLC,and the uptake per unit area in different groups was analyzed by One-way ANOVA.Result:Compared with the normal J70 group(in normal environment,the concentration of berberine hydrochloride was 70 mg·L-1)at the same time point,the uptake per unit area of the normal J70+Ver100 group(in normal environment,the concentration of berberine hydrochloride was 70 mg·L-1,adding verapamil hydrochloride to a concentration of 100 mg·L-1)was significantly increased in the ileum(P<0.05)at 120 min;the uptake per unit area values of the high calcium J70+A35 group(in high calcium environment,the concentration of berberine hydrochloride was 70 mg·L-1,adding berbamine hydrochloride to a concentration of 35 mg·L-1)were significantly increased in the duodenum(P<0.05)at 30,60,90 min;and the uptake per unit area values of the high calcium J70+A70 group(in high calcium environment,the concentration of berberine hydrochloride was 70 mg·L-1,adding berbamine hydrochloride to a concentration of 70 mg·L-1)were significantly increased in the ileum(P<0.05)at 30,90,120 min.By comparing with the normal group with the same mass concentration,the intestinal absorption of berberine hydrochloride was better in the high calcium J70+A35 group and the high calcium J70+A70 group.Conclusion:Berbamine hydrochloride can promote the absorption of berberine hydrochloride in intestine to a certain extent,especially in the high calcium environment.
作者
向宇楠
王小艳
冯慧
赵娅
李啟恩
李先加
赖先荣
XIANG Yu-nan;WANG Xiao-yan;FENG Hui;ZHAO Ya;LI Qi-en;LI Xian-jia;LAI Xian-rong(College of Pharmacy,School of Ethnic Medicine,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;Tibetan Medical College,Qinghai University,Xining 810000,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2019年第17期98-103,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金面上项目(81774007,81173360,81560806)
青海省科技计划项目(2017-ZJ-922Q)
关键词
盐酸小檗碱
盐酸小檗胺
外翻肠囊法
高效液相色谱法
肠吸收
P-糖蛋白
高钙环境
berberine hydrochloride
berbamine hydrochloride
everted intestinal sac method
high performance liquid chromatography(HPLC)
intestinal absorption
P-glycoprotein
high calcium environment
作者简介
第一作者:向宇楠,在读硕士,从事中药及民族药创新药物研究,E-mail:1206106551@qq.com;通讯作者:赖先荣,教授,从事中药及民族药创新药物研究,Tel:028-61656141,E-mail:vegf@qq.com;通讯作者:李先加,教授,从事藏族医药防治重大疾病及特色优势病种研究,Tel:0971-5310695,E-mail:lxgyal@sina.com
