摘要
利用链脲佐菌素（ＳＴＺ）引起的大鼠糖尿病性实验性白内障模型，观察小檗胺对晶状体上皮细胞ＤＮＡ损伤、修复及致障过程的影响．发现ＳＴＺ对照组在腹腔注射ＳＴＺ后３～４ｄ开始出现有显著意义的ＤＮＡ单链断裂（ｓｉｎｇｌｅｓｔｒａｎｄｂｒｅａｋｓ，ＳＳＢ），并持续存在于发病全程直至晶状体完全混浊．而在注射ＳＴＺ后１２ｈ再腹腔注射３．４８ｍｇ／ｋｇ体重，１．７４ｍｇ／ｋｇ体重小檗胺后，一周后才出现有显著意义的ＳＳＢ．３．４８ｍｇ／ｋｇ体重组在５周后白内障形成率明显低于ＳＴＺ组的同时，ＳＳＢ也恢复到对照水平，而１．７４ｍｇ／ｋｇ体重组第７周才恢复到对照水平．提示抗氧化药物小檗胺能在一定程度上阻止白内障发生过程中的ＤＮＡ损伤．
The effect of berbamine,a monomer extracted from a Chinese herb (an antioxidative and a free radical scavenger),on DNA damage/repair in cataractogenesis of diabetic cataract induced by streptozotocin(STZ) was studied.The results showed that 348mg/kg b. wt. of berbamine could inhibit or delay the appearance of DNA damage (SSB) and accelerated DNA repair(as well as decrease the incidence of cataract).While 174 mg/kg b. wt. of berbamine was not as effective as that of 348 mg/kg b. wt. of berbamine.SSB appeared very early and continued into opaque phase and a positive correlation between SSB and lens opacity was showed.Oxidative damage may be one of the most important mechanisms in diabetic cataractogenesis.Berbamine has the preventive effect on cataractogenesis.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
1998年第3期300-303,共4页
Chinese Journal of Biochemistry and Molecular Biology
基金
国家自然科学基金
关键词
DNA损伤
小檗胺
糖尿病
白内障
病理学
DNA damage/repair
Single strand breaks
Berbamine
Diabetic cataract
Streptozotocin
