摘要
目的建立易黄汤物质基准的最佳制备工艺。方法采用单因素试验法和正交试验法,以小檗碱、没食子酸、毛蕊花糖苷和固含物的转移率为评价指标,采用HPLC法测定含量,对易黄汤前处理、煎煮、过滤、浓缩和干燥等工序的参数进行优选。结果易黄汤物质基准最优的制备工艺为:取炒白果粉碎过1号筛,酒车前子包煎,同其它饮片置于药罐中煎煮2次,第1次加8倍量水,浸泡30 min,加盖煎煮30 min;第2次取药渣再加7倍量水煎煮25 min,趁热滤过。合并两次滤液减压浓缩至1.0 g/mL饮片,冷冻干燥48 h,得冻干粉约8 g,置于20 mL棕色玻璃西林瓶中密封保存,即得易黄汤物质基准。结论优选的易黄汤物质基准制备工艺稳定可行。
Objective To establish the best preparation process of Yihuang Decoction(易黄汤)material standard.Methods Single factor test method and orthogonal test method were used to evaluate the transfer rate of berberine,gallic acid,verbascoside and solid content.The content was determined by HPLC method.The pretreatment,decoction and filtration of Yihuang Decoction,concentration and drying process parameters were optimized.Results The optimal preparation process of Yihuang Decoction material standard were:take the fried ginkgo and crush it through the No.1 sieve,and fry the psyllium psyllium for frying,then place it in a medicine pot with other decoction pieces for two times,add 8 times the amount of water for the first time,soak for 30 minutes,cover and decoct for 30 minutes,take the medicine residue for the second time and add 7 times the amount of water to decoct for 25 minutes.The two filtrates were combined under reduced pressure and concentrated to 1.0 g/mL,and freeze-dried for 48 hours to obtain about 8 g of freezedried powder,which was placed in a 20 mL brown glass vial and seal and store to obtain the substance standard of Yihuang Decoction.Conclusion The optimal preparation process of Yihuang Decoction is stable and feasible.
作者
王琪
何瑞欣
徐雅蝶
张晓云
黄仕文
嵇晶
程建明
刘陶世
WANG Qi;HE Ruixin;XU Yadie;ZHANG Xiaoyun;HUANG Shiwen;JI Jing;CHENG Jianming;LIU Taoshi(Nanjing University of Chinese Medicine,Nanjing 210023,Jiangsu,China;Jiangsu Key Laboratory of Effective Substances of Chinese Medicine,Nanjing 210023,Jiangsu,China)
出处
《辽宁中医药大学学报》
CAS
2021年第11期43-48,共6页
Journal of Liaoning University of Traditional Chinese Medicine
基金
江苏省科技厅科技项目面上项目(BK20181424)
南京同仁堂经典名方产品研发项目(2018053,2018054)
关键词
易黄汤
物质基准
小檗碱
没食子酸
毛蕊花糖苷
Yihuang Decoction(易黄汤)
material standard
berberine
gallic acid
verbascoside
作者简介
王琪(1995-),女,河北沧州人,硕士研究生,研究方向:中药制药技术与开发研究;通讯作者:刘陶世(1971-),男,湖南邵阳人,副研究员,博士,研究方向:中药药剂学研究。E-mail:tsliur4111@sina.com;通讯作者:程建明(1965-),男,江苏淮安人,研究员,博士,研究方向:中药新剂型、新技术研究及其产品开发。E-mail:cjm7895@163.com。
