摘要
目的探讨复方片仔癀肝宝(GB)对急性肝损伤的保护作用。方法将60只SD大鼠随机分为正常组、模型组、西利宾胺组(阳性对照)、GB低剂量组、GB中剂量组、GB高剂量组,每组10只。连续给药7 d后,采用CCl4诱导急性肝损伤模型,24 h后检测GB对ALT、AST、ALP的影响;HE染色观察GB对肝组织病理学的影响;Elisa法检测GB对TNF-α和IL-1β的影响。结果各实验组ALT、AST、ALP的活性显著降低,观察肝脏病理切片各实验组与模型组比较,肝损伤均有减轻的趋势;血清中TNF-α和IL-1β的含量降低,差异有统计学意义(P<0.05)。结论 GB能显著减轻CCl4诱导的急性肝损伤,可能与GB的抗炎症反应有关。
Objective To explore the protective action of complex prescription Pianzaihuang Ganbao on acute liver injury. Methods Sixty Sprague Dawley rats were divided into six groups randomly as follows:control group,the model group,Silymarin group( positive control),Ganbao( GB) low dose group,GB middle dose group,GB high dose group. The rats were injected carbon tetrachloride( CCl4) subcutaneously to establish the acute liver injury model,except for the rats in control group. Rats in therapeutic groups were pretreated with GB and silymarin seven days before CCl4 was given. After 24 hours,serum was obtained to determine the activity of ALT、AST and ALP. Hematoxylin- Eosin( HE) staining was used to detect the pathological change. Enzyme linked immunosorbent assay( Elisa) was used to explore the expression of TNF- αand IL-1β. Results GB and silymarin could inhibit the activity of ALT,AST and ALP. Both GB and silymarin could ameliorate the pathology of liver and decrease the expression of TNF- αand IL- 1β. Conclusion GB could relieve the liver injury induced by CCl4,maybe correlate with its anti- inflammation activity.
出处
《世界中西医结合杂志》
2015年第9期1216-1218,共3页
World Journal of Integrated Traditional and Western Medicine
基金
福建省科技厅项目(2010YZ0001-1)
关键词
复方片仔癀肝宝
急性肝损伤
保护作用
Complex Prescription Pianzaihuang Ganbao
Acute liver Injury
Protective effect
