摘要
目的:探讨依达拉奉右莰醇对脑出血(intracerebral hemorrhage,ICH)血肿周围脑组织病理损伤的防治作用及其可能机制。方法:将176只SD大鼠随机分为假手术组、ICH组、依达拉奉组和依达拉奉右崁醇组,每组44只,后3组构建急性ICH模型,每组按评估时间分为1、3、7和14 d 4个小组,每个小组11只。造模2 h后腹腔注射给药:假手术组和ICH组(生理盐水6 mL/kg)、依达拉奉组(依达拉奉6 mg/kg)、依达拉奉右莰醇组(依达拉奉右莰醇7.5 mg/kg),随后每12 h给药1次直至14 d。各组大鼠分别采用mNSS评分标准进行神经功能评分,干湿法测定脑组织含水量,生化法测定血肿周围脑组织一氧化氮(nitric oxide,NO)和总抗氧化能力(total antioxidant capacity,T-AOC),免疫组化法测定血肿周围脑组织CD16、CD206含量。结果:假手术组、ICH组、依达拉奉组、依达拉奉右莰醇组在3 d(病情高峰期)时间点的神经功能评分分别为:(0.6±0.5)、(14.0±1.6)、(9.8±0.8)、(10.4±1.1);脑含水量(%)分别为:(63.2±2.14)、(85.61±1.43)、(81.48±1.9)、(76.77±1.44);NO含量(单位μmol/gprot)分别为:(2.45±0.46)、(9.98±0.54)、(8.77±0.36)、(7.92±0.43);T-AOC含量(单位mM/mgprot)分别为:(0.67±0.02)、(0.74±0.03)、(0.78±0.02)、(0.84±0.03);CD16含量(单位iod值定量)分别为(143.8±15.82)、(3673.81±166.33)、(2970.74±132.75)、(2521.69±140.74);CD206含量(单位iod值定量)分别为:(548.46±93.68)、(726±97.81)、(915.28±100.33)、(1119.51±160.52);依达拉奉右茨醇组的上述指标与假手术组、ICH组、依达拉奉组相比,差异均有统计学意义(均P<0.05)。结论:依达拉奉右莰醇可以减轻脑出血所致的神经功能缺损,其机制可能是通过减轻血肿周围脑组织水肿及氧化应激,调节小胶质细胞的极化而发挥神经保护作用。
Objective:To investigate the role and possible mechanism of edaravone dexborneol in the prevention and control of patho-logical injury of brain tissue around hematoma after intracerebral hemorrhage(ICH).Methods:A total of 176 Sprague-Dawley rats were randomly divided into sham-operation group,ICH group,edaravone group,and edaravone dexborneol group,with 44 rats in each group.All rats except those in the sham-operation group were used to establish a rat model of acute ICH,and each group was further di-vided into 1-day,3-day,7-day,and 14-day subgroups according to the time of evaluation,with 11 rats in each subgroup.At 2 hours after modeling,drugs were administered via intraperitoneal injection,i.e.,6 ml/kg normal saline for the sham-operation group and the ICH group,6 mg/kg edaravone for the edaravone group,and 7.5 mg/kg edaravone dexborneol for the edaravone dexborneol group,fol-lowed by subsequent administration every 12 hours until day 14.The modified Neurological Severity Score(mNSS)was used to assess neurological function;the wet-dry weight method was used to measure the water content of brain tissue;the biochemical methods were used to measure nitric oxide(NO)and total antioxidant capacity(T-AOC)in brain tissue around the hematoma;immunohistochemistry was used to measure the content of CD16 and CD206 in brain tissue around the hematoma.Results:The mNSS score on day 3(peak of the disease)was 0.6±0.5 for the sham-operation group,14.0±1.6 for the ICH group,9.8±0.8 for the edaravone group,and 10.4±1.1 for the edaravone dexborneol group,with a water content of 63.2±2.14,85.61±1.43,81.48±1.9,and 76.77±1.44,respectively,and the content of NO was 2.45±0.46,9.98±0.54,8.77±0.36,and 7.92±0.43,respectively;the content of T-AOC was 0.67±0.02,0.74±0.03,0.78±0.02,and 0.84±0.03,respectively;the content of CD16 was 143.8±15.82,3673.81±166.33,2970.74±132.75,and 2521.69±140.74,respectively;the content of CD206 was 548.46±93.68,726±97.81,915.28±100.33,and 1119.51±160.52,respectively.There were significant differences in these indica-tors between the edaravone dexborneol group and the other three groups(all P<0.05).Conclusion:Edaravone dexborneol can alleviate neurological deficit caused by ICH and exert a neuroprotective effect by reducing brain tissue edema around the hematoma,alleviating oxidative stress,and regulating the polarization of microglial cells.
作者
吴芝璇
王茂娟
李作孝
Wu Zhixuan;Wang Maojuan;Li Zuoxiao(Department of Neurology,Affiliated Hospital of Southwest Medical University;Department of Critical Care Medicine,Deyang People’s Hospital)
出处
《重庆医科大学学报》
北大核心
2025年第8期1029-1034,共6页
Journal of Chongqing Medical University
基金
泸州市人民政府-西南医科大学科技战略合作基金资助项目(编号:2018LZXNYD-ZK17)。
关键词
依达拉奉右莰醇
依达拉奉
脑出血
氧化应激
小胶质细胞极化
edaravone dexborneol
edaravone
intracerebral hemorrhage
oxidative stress
microglial cell polarization
作者简介
吴芝璇,Email:80422722@qq.com,研究方向:神经免疫。;通信作者:李作孝,Email:1zx3235@sina.com。
