摘要
为明确螺虫乙酯在枸杞Lycium上的残留特性,在我国宁夏、新疆、河北和青海4地开展了22.4%螺虫乙酯悬浮剂的田间残留试验。优化了净化条件,建立了枸杞中螺虫乙酯及其代谢物残留的液相色谱三重四极杆串联质谱检测方法。在0.01~1.00 mg/kg范围内,螺虫乙酯及其代谢物标准曲线方程的线性关系良好,r>0.999,基质效应为14.3%~107.1%;方法定量限为0.01 mg/kg。在0.01~1.00 mg/kg的添加范围内,螺虫乙酯及其代谢物在枸杞上的平均回收率为71.8%~106.2%,相对标准偏差(relative standard deviation,RSD)为1.9%~9.1%。12个月内,螺虫乙酯及其代谢物在枸杞上的降解率均低于30%,稳定性试验结果表明螺虫乙酯及其代谢物在枸杞上是稳定的。枸杞中螺虫乙酯的残留消解动态符合一级动力学模型,在新疆与宁夏的降解半衰期为1.5 d和2.3 d。最终残留结果显示,施药1次,安全间隔期为7 d时,螺虫乙酯及其代谢物的残留量为0.01~0.24 mg/kg,低于我国制定的临时最大残留限量值5 mg/kg。风险评估结果表明,螺虫乙酯在枸杞上的风险商为26.3%,风险处于可接受水平。
To investigate the residue behavior and underlying risks of spirotetramat in Lycium,field residue trials of 22.4%spirotetramat suspension concentrate(SC)were conducted in four provinces of China:Ningxia,Xinjiang,Hebei and Qinghai.A liquid chromatography-tandem mass spectrometry(LC-MS/MS)method was developed for detecting spirotetramat and its metabolites,with optimized purification procedures.Within the concentration range of 0.01-1.00 mg/kg,the calibration curves showed good linearity(r>0.999),and matrix effects ranged from 14.3%to 107.1%.The limit of quantitation was 0.01 mg/kg.At spiking levels between 0.01-1.00 mg/kg,the average recoveries were 71.8%-106.2%,with the relative standard deviations(RSD)between 1.9%and 9.1%.After 12 months of storage,the degradation rates of spirotetramat and its metabolites in berries of Lycium were all below 30%,indicating good storage stability.The dissipation of spirotetramat followed a first-order kinetic model,with half-lives in Xinjiang and Ningxia of 1.5 d and 2.3 d.The final residual results showed that following a single application and a 7-day pre-harvest interval,the residual quantity of spirotetramat and its metabolites in Lycium was 0.01-0.24 mg/kg,which was lower than China's provisional maximum residue limit of 5 mg/kg.Dietary risk assessment showed a risk quotient of 26.3%,indicating that dietary exposure to spirotetramat through Lycium is within acceptable safety margins.
作者
杨静
马小龙
魏新举
王晓菁
苟春林
YANG Jing;MA Xiaolong;WEI Xinju;WANG Xiaojing;GOU Chunlin(Institute of Quality Standard and Testing Technology for Agro-Products of Ningxia,Key Laboratory of Agricultural Product Quality and Safety Standards Research and Evaluation of Ningxia Hui Autonomous Region,Yinchuan 750002,China)
出处
《植物保护》
北大核心
2025年第4期266-274,共9页
Plant Protection
基金
“十四五”农业高质量发展和生态保护科技创新示范项目(NGSB-2021-5-03)
2022宁夏回族自治区科技创新领军人才项目(2022GKLRLX09)。
关键词
枸杞
螺虫乙酯
稳定性
残留
膳食风险评估
Lycium
spirotetramat
stability
residue
dietary risk assessment
作者简介
通信作者:苟春林,E-mail:48113526@qq.com。
