摘要
为研究染色体加倍对水稻耐碱性的影响,以二倍体水稻(93-11D)与四倍体水稻(93-11T)为材料,利用pH为11.39的NaOH溶液处理水稻幼苗,并检测碱胁迫下的过氧化物酶(POD)和超氧化物歧化酶(SOD)活性以及相关基因的表达量,探讨染色体加倍对水稻耐碱能力的影响。表型观测结果显示,93-11D和93-11T在碱溶液胁迫24 h时均有萎蔫现象,但93-11T强于93-11D。SOD和POD活性测定结果表明:93-11D水稻幼苗SOD呈先下降后上升趋势,93-11T水稻幼苗SOD呈上升趋势,93-11D和93-11T POD整体呈振荡下降趋势;从SOD、POD活性变化量来看,93-11T和93-11D整体相差不大;从应激反应速度来看,93-11T响应碱胁迫的速度比93-11D快。基因表达检测结果显示,不同倍性水稻中SOD、POD相关基因在受碱胁迫后基因表达量不同,尤其是染色体加倍后的93-11T中相关基因在胁迫24 h时相比93-11D表达量显著增加,且基因表达在根和叶不同器官中表达差异也较明显。SOD、POD相关基因与抗氧化系统密切相关,基因表达的上调使其抗氧化能力增强,植株以此来抵御碱胁迫。染色体加倍是产生植物多样性的重要方式之一,还需进一步研究不同倍性水稻应对碱胁迫反应的分子机制,为深入探索染色体加倍对水稻耐碱性分子机制奠定基础。
In order to study the effects of chromosome doubling on alkaline tolerance of rice,rice seedlings were treated with NaOH solution at pH 11.39 using diploid rice(93-11D)and tetraploid rice(93-11T)as materials.The activities of peroxidase(POD)and superoxide dismutase(SOD)and the expression levels of related genes under alkali stress were detected,and the effects of chromosome doubling on alkali tolerance of rice were discussed.Phenotypic observation showed that both 93-11D and 93-11T showed wilting at 24 h under alkaline solution stress,but 93-11T was stronger than 93-11D.The results of SOD and POD activity measurement showed that SOD decreased first and then increased in rice seedlings at 93-11D,SOD increased in rice seedlings at 93-11T,and POD oscillated downward in rice seedlings at 93-11D and 93-11T.In terms of changes in SOD and POD activities,there was no significant difference between 93-11T and 93-11D.In terms of stress response speed,93-11T responded to alkali stress faster than 93-11D.The results of gene expression detection showed that SOD and POD gene expression levels were different in different ploidy rice under alkali stress,especially in 93-11T after chromosome doubling.
作者
李喆
卢凤丹
王薇
王宁宁
马建
LI Zhe;LU Fengdan;WANG Wei;WANG Ningning;MA Jian(College of Agronomy,Jilin Agricultural University,Changchun 130118,China)
出处
《吉林农业大学学报》
北大核心
2025年第3期392-399,共8页
Journal of Jilin Agricultural University
基金
国家重点研发计划项目(2016YFD0102000)。
作者简介
李喆,女,在读硕士,研究方向:生物技术在作物遗传育种上的应用;通信作者:王宁宁,E-mail:ningningw@jlau.edu.cn;通信作者:马建,E-mail:majian197916@jlau.edu.cn。
