摘要
四倍体栽培种花生是由二倍体野花生进化而来的。多倍化过程中,多倍体的基因库扩大,同时基因可能产生多种功能。因此,对不同倍性花生的基因进行分析,有利于研究多倍化过程中基因表达调控网络的变化,为花生育种提供理论指导。花生线粒体转录终止因子AhmTERF1与其二倍体同源基因Aradu.QVX65的CDS序列高度相似,但启动子序列差异大。启动子的差异可能影响基因在不同倍性花生中的表达水平。为了进一步探究二者的启动子差异,本研究采用PLACE数据库进行顺式作用元件预测,与Aradu.QVX65启动子相比,AhmTERF1启动子调控区含有更丰富的顺式作用元件,包括多种组织特异性相关元件和胁迫响应元件。并且AhmTERF1启动子包含一段CT-rich序列。通过构建完整和缺失CT-rich序列的GUS报告载体,发现CT-rich序列可增强AhmTERF1的表达。推测AhmTERF1和Aradu.QVX65启动子序列差异,可能导致了在不同倍性花生中,基因的功能有不同。本研究加深了对花生多倍化过程中序列变化的认识,从分子水平探讨基因表达调控的机制,为基于启动子调控的作物遗传改良提供新思路。
Cultivated peanut is an allotetraploid species whose ancestral genomes are probably derived from wild Arachis species.Polyploidization events resulted in gene pool expanded and multi-functionality of genes in allotetraploid species.Thus,the analysis of genes in different ploidy peanut is helpful to study the changes of gene expression regulation network in the process of polyploidy and provide theoretical guidance for peanut breeding.The CDS sequence of peanut mitochondrial transcription termination factor AhmTERF1 shares high similarity with that of diploid homologous gene Aradu.QVX65,but the promoters differ greatly,which may affect gene expression in different ploidy peanuts.In order to explore the differences of promoters,the cis-acting elements were predicted by the PLACE database for sequences analysis.The prediction results of cis-acting elements showed that AhmTERF1 promoter contains more cis-acting elements compared with Aradu.QVX65 promoter,including tissue-specific elements and stress-responsive elements.And AhmTERF1 promoter contains a CT-rich sequence.Further,through the GUS reporter gene,it was found that CT-rich sequence enhances the expression of AhmTERF1.It is speculated that the promoter sequences of AhmTERF1 and Aradu.QVX65 are different,which may lead to different gene functions in diploid and allotetraploid peanuts.These study deepen the understanding of sequence changes during peanut polyploidization,explore the mechanism of gene expression regulation at the molecular level and provide a new idea for crop genetic improvement based on promoter regulation.
作者
李丽梅
李玲
李晓云
Li Limei;Li Ling;Li Xiaoyun(Life Sciences College,Zhaoqing University,Zhaoqing,526061;Guangdong Provincial Key Laboratory of Biotechnology for Plant Development,School of Life Science,South China Normal University,Guangzhou,510631)
出处
《分子植物育种》
北大核心
2025年第14期4592-4598,共7页
Molecular Plant Breeding
基金
国家自然科学基金项目(32071924)
肇庆学院博士启动基金项目(210073)共同资助。
作者简介
通信作者:李晓云,20185131@m.scnu.edu.cn。
