摘要
将八味沉香丸组方药材分别经水回流提取,70%乙醇回流提取,70%乙醇回流提取后再以纯水提取,得到3种提取物(ST、CT、CST);采用小鼠急性心肌缺血(AMI)和对氯苯基丙氨酸(PCPA)致小鼠失眠2种模型,分别对ST(760 mg·kg^(-1))、CT(620 mg·kg^(-1))、CST(1 040 mg·kg^(-1))进行药效评价,并采用Western blot探索其助眠作用机制;采用UPLC-Q-Exactive-MS技术鉴定不同提取物特征时段的主要化学成分。结果显示,CT和CST显著提高心肌梗死小鼠的心脏射血分数(EF)和短轴缩短率(FS),降低左室舒张末内径(LVIDd)和左室收缩末内径(LVIDs);ST在各指标中均无显著改善。CT显著缩短失眠小鼠睡眠潜伏期,延长睡眠维持时间;ST未缩短睡眠潜伏期,但显著延长了睡眠维持时间;CST未缩短小鼠睡眠潜伏期,也未延长小鼠睡眠维持时间;CT和ST均能上调脑组织谷氨酸脱羧酶67(GAD67)蛋白的表达。从CT中共鉴定出2-(2-苯乙基)色酮、6-甲氧基-2-(2-苯乙基)色酮等15个主要化学成分,从ST中共鉴定出诃子次酸等6个化学成分。该结果提示色酮及萜类为八味沉香丸潜在的抗心肌缺血药效物质,鞣质、酚酸类为潜在的助眠药效物质。该研究丰富了八味沉香丸的药理和化学研究,为其二次开发、质量标准提升和临床应用提供了依据和参考。
The medicinal materials of Bawei Chenxiang Pills(BCPs)were extracted via three methods:reflux extraction by water,reflux extraction by 70%ethanol,and extraction by pure water following reflux extraction by 70%ethanol,yielding three extracts of ST,CT,and CST.The efficacy of ST(760 mg·kg^(-1)),CT(620 mg·kg^(-1)),and CST(1040 mg·kg^(-1))were evaluated by acute myocardial ischemia(AMI)and p-chlorophenylalanine(PCPA)-induced insomnia in mice,respectively.Western blot was further utilized to investigate their hypnosis mechanisms.The main chemical components of different extracts were identified by the UPLC-Q-Exactive-MS technique.The results showed that CT and CST significantly increased the ejection fraction(EF)and fractional shortening(FS)of myocardial infarction mice,reduced left ventricular internal dimension at end-diastole(LVIDd)and left ventricular internal dimension at end-systole(LVIDs).In contrast,ST did not exhibit significant effects on these parameters.In the insomnia model,CT significantly reduced sleep latency and prolonged sleep duration,whereas ST only prolonged sleep duration without shortening sleep latency.CST showed no significant effects on either sleep latency or sleep duration.Additionally,both CT and ST upregulated glutamic acid decarboxylase 67(GAD67)protein expression in brain tissue.A total of 15 main chemical components were identified from CT,including 2-(2-phenylethyl)chromone and 6-methoxy-2-(2-phenylethyl)chromone.Six chemical components including chebulidic acid were identified from ST.The results suggested that chromones and terpenes were potential anti-myocardial ischemia drugs of BCPs,and tannin and phenolic acids were potential hypnosis drugs.This study enriches the pharmacological and chemical research of BCPs,providing a basis and reference for their secondary development,quality standard improvement,and clinical application.
作者
王嘉童
康露璐
周凤
格桑罗布
梁亚娜
杨国栋
高小力
武慧超
柴兴云
WANG Jia-tong;KANG Lu-lu;ZHOU Feng;GESANG Luo-bu;LIANG Ya-na;YANG Guo-dong;GAO Xiao-li;WU Hui-chao;CHAI Xing-yun(Modern Research Center of Chinese Medicine,Beijing Academy of Traditional Chinese Medicine,Bejing University of Chinese Medicine,Beijing 102488,China;Xizang Ganlu Pharmaceutical Technology Limited Liability Company,Lhasa 850000,China;Institute of Ethnic Medicine,School of Chinese Medicine,Bejing University of Chinese Medicine,Beijing 102488,China)
出处
《中国中药杂志》
北大核心
2025年第11期3035-3042,共8页
China Journal of Chinese Materia Medica
基金
国家自然科学基金青年科学基金项目(82204763)
西藏自治区科技计划重点研发计划项目(XZ202201ZY0009G)。
关键词
八味沉香丸
抗心肌缺血
助眠
药效物质
Bawei Chenxiang Pills
anti-myocardial ischemia
hypnosis
pharmacodynamic substance
作者简介
通信作者:柴兴云,研究员,主要从事民族药药效物质研究,Tel/Fax:(010)64286350,E-mail:xingyunchai@yeah.net;通信作者:武慧超,副研究员,主要从事民族药新剂型与新技术研究,Tel/Fax:(010)64286010,E-mail:wuhuichao@bucm.edu.cn;王嘉童,硕士研究生,E-mail:wjkid555781@163.com。
