摘要
以寄生于梭梭与四翅滨藜2种寄主的肉苁蓉为试材,采用苯酚-硫酸法测定2种寄主肉苁蓉多糖含量,并通过Illumina Novaseq 6000测序平台对梭梭-肉苁蓉与四翅滨藜-肉苁蓉的转录组进行测序分析,研究不同寄主肉苁蓉转录组差异,筛选多糖代谢过程中相关酶基因,并对筛选所得基因进行qRT-PCR验证,以期为肉苁蓉新寄主的挖掘与遗传学改良提供参考依据。结果表明:四翅滨藜-肉苁蓉的多糖含量高于梭梭-肉苁蓉,通过转录组比较分析,从四翅滨藜-肉苁蓉与梭梭-肉苁蓉肉质茎中共筛选得到14089个差异表达基因(DEGs),KEGG富集结果发现DEGs在碳水化合物代谢、半乳糖代谢、氨基酸及脂肪酸代谢等途径中富集最为显著,此外,共获得4条多糖代谢相关途径上的26个DEGs,其中,β-果糖苷酶基因、β-半乳糖苷酶基因在梭梭-肉苁蓉的表达量显著高于四翅滨藜-肉苁蓉;UDP-葡萄糖脱氢酶基因、甘露糖-1-磷酸鸟苷酸转移酶基因在四翅滨藜-肉苁蓉的表达量显著高于梭梭-肉苁蓉,以上基因可能是调控肉苁蓉多糖代谢差异的关键酶基因。
Taking Cistanche deserticola parasitizing two host plants(Haloxylon ammodendron and Atriplex canescens)as the test materials,this study determined the polysaccharide content in C.deserticola from different hosts using the phenol-sulfuric acid method.Transcriptome sequencing and analysis of Haloxylon ammdendron-C.deserticola and Atriplex canescens-C.deserticola associations were conducted using the Illumina NovaSeq 6000 sequencing platform.The research focused on investigating transcriptome differences in C.deserticola from different hosts,screening key enzymatic genes involved in polysaccharide metabolism,and validating the selected genes through qRT-PCR verification,in order to provide a reference for exploring new host plants and genetic improvement of C.deserticola.The results showed that the polysaccharide content found in Atriplex canescens-C.deserticola was superior to that present in Haloxylon ammodendron-C.deserticola.Through comparative analysis of transcriptome,14089 differentially expressed genes(DEGs)were screened from the fleshy stems of Atriplex canescens-C.deserticola and Haloxylon ammodendron-C.deserticola.The results of KEGG enrichment analysis revealed that these DEGs were most prominently enriched in pathways related to carbohydrate metabolism,galactose metabolism,amino acid metabolism,and fatty acid metabolism.Furthermore,a total of 26 DEGs were obtained from four pathways related to polysaccharide metabolism,among which the expression levels ofβ-fructosidase gene andβ-galactosidase gene in Haloxylon ammodendron-C.deserticola were significantly higher than those in Atriplex canescens-C.deserticola.The expression levels of UDP-glucose dehydrogenase gene and mannose-1-phosphate guanylate transferase gene in Atriplex canescens-C.deserticola were significantly higher than those in Haloxylon ammodendron-C.deserticola,which may be the key enzyme genes for regulating the difference of polysaccharide metabolism in C.deserticola.
作者
张文瑶
郭晔红
吕霞
陈锦辉
贾存勤
ZHANG Wenyao;GUO Yehong;LYU Xia;CHEN Jinhui;JIA Cunqin(College of Agronomy,Gansu Agricultural University/State Key Laboratory of Aridland Crop Science,Lanzhou,Gansu 730070;Gansu Huiqin Biological Technology Co.Ltd.,Baiyin,Gansu 730600)
出处
《北方园艺》
北大核心
2025年第9期35-43,共9页
Northern Horticulture
基金
国家自然科学基金资助项目(31860349)
甘肃省科技计划资助项目(18YFlNA072)。
关键词
肉苁蓉
转录组测序
寄主
多糖代谢差异
Cistanche deserticola
transcriptome sequencing
hosts
difference of polysaccharide metabolism
作者简介
第一作者:张文瑶(1999-),男,硕士研究生,研究方向为中药学。E-mail:1623711094@qq.com;郭晔红(1968-),女,博士,教授,博士生导师,现主要从事药用植物资源开发与利用等研究工作。E-mail:guoyh@gsau.edu.cn.
