摘要
目的研究冯了性风湿跌打药酒在大鼠体内的入血原型成分及代谢产物。方法采用超高效液相色谱-四极杆/静电场轨道阱高分辨质谱法(UHPLC-Q Exactive Focus MS/MS)技术,通过比对冯了性风湿跌打药酒液、空白血清和含药血清的图谱差异,根据保留时间、相对分子质量,以及一级、二级离子碎片,解析大鼠灌胃冯了性风湿跌打药酒后血清中的原型成分和代谢产物。检测条件:流动相选择甲醇(A)-0.1%甲酸水溶液(B)梯度洗脱,流速0.3 ml/min,加热电喷雾离子源,检测范围m/z 80~1200,正、负离子扫描模式。结果共检测到血清中31个移行成分,其中9个为原型成分,22个为代谢产物。经鉴定9个原型成分分别为苯乙醛、包公藤丙素/凹脉丁公藤碱、对香豆酸、5-羟甲基糠醛、奎尼酸、丹皮酚、3-羟基苯甲醛、水杨酸、异莪术醇,22个代谢产物主要为11个有机酸类成分、3个吲哚类成分、2个有机酚类成分、2个生物碱类成分、1个核苷类成分、1个氨基酸类成分、1个内酯类成分、1个磺基类成分,其代谢途径主要为葡萄糖醛酸化、硫酸酯化等,以Ⅱ相代谢为主。结论有机酚类和有机酸类是冯了性风湿跌打药酒进入体内的主要成分,生物碱类化合物、有机酸类成分可能是其发挥药效的潜在活性成分。
Objective To analyze the blood-transition prototype components and metabolites of Fengliaoxing Fengshi Dieda medicinal liquor.Methods Ultra-high performance liquid chromatographyquadrupole/electrostatic field orbital trap high resolution mass spectrometry(UHPLC-Q Exactive Focus MS/MS)technique was used to compare the chromatogram differences of Fengliaoxing Fengshi Dieda medicinal liquor extract,blank serum and drug-containing serum.According to the retention time,relative molecular weight and the ratio with primary and secondary ion fragments provided by MS,the prototype components and metabolites of Fengliaoxing Fengshi Dieda medicinal liquor extract were analyzed in serum of rats after oral administration.The detection conditions were as follows:the mobile phase of methanol(A)-0.1%formic acid solution(B)for elution gradient(0-5 min,5%A;5-60 min,5%-95%A;60-65 min,95%A),the flow rate of 0.3 ml/min,heated electrospray ionization,detection range of m/z 80-1200,positive and negative ion scanning modes.Results A total of 31 transitional components were detected in the serum,of which 9 were prototype components and 22 were metabolites.The 9 prototype components were identified as phenylacetaldehyde,baogongteng C/erycibellin,p-coumaric acid,5-Hydroxymethylfurfural,quinic acid,paeonol,3-Hydroxybenzaldehyde,salicylic acid,and isourecumenol.The 22 metabolites mainly consist of 11 organic acid components,3 indole components,2 organic phenolic components,2 alkaloid components,1 nucleoside component,1 amino acid component,1 lactone component,and 1 sulfonic component.The metabolic pathways were mainly glucuronidation,sulfation and others,which by phaseⅡmetabolism.Conclusion Organic phenols and organic acids are the main components that enter the body of Fengliaoxing Fengshi Dieda Medicinal Liquor,while alkaloid compounds and organic acid components may be potential active ingredients for its pharmacological effects.
作者
曲彤
李宁
任慧
鲁文静
崔小敏
胡静
陈志永
Qu Tong;Li Ning;Ren Hui;Lu Wenjing;Cui Xiaomin;Hu Jing;Chen Zhiyong(Institute of Chinese Materia Medica,Shaanxi Academy of Traditional Chinese Medicine,Xi'an 710003,China)
出处
《国际中医中药杂志》
2025年第3期357-363,共7页
International Journal of Traditional Chinese Medicine
基金
国家自然科学基金(81973419)
“陕西省中医药管理局双链融合”中青年科研创新团队(2022-SLRH-YQ-003)
陕西省中医药管理局“医研校企”中医药传承创新平台(2023年)。
关键词
冯了性风湿跌打药酒
血清药物化学
原型成分
代谢产物
Feng Liao Xing Feng Shi Die Da Yao Jiu
Serum pharmacochemistry
Prototype components
Metabolites
作者简介
通信作者:陈志永,Email:18829014325@163.com。
