摘要
目的:研究肝郁气滞型三阴性乳腺癌(Triple Negative Breast Cancer,TNBC)患者肠道菌群的多样性与差异性。方法:根据纳入与排除标准纳入肝郁气滞型TNBC患者8例作为观察组,同时纳入健康志愿者10例作为对照组,分别收集其粪便标本。采用16S rDNA测序技术检测观察组与对照组的肠道菌群丰度及菌群分布情况,并通过组间差异分析寻找具有显著性差异(P<0.05)的菌属。结果:观察组与对照组的肠道菌群多样性显著、物种丰富,且两组肠道菌群分布存在显著差异(P<0.05),其中观察组较对照组增多的物种有解黄酮菌属、变形菌属、水栖菌属、柠檬酸杆菌属、厌氧球形菌属、醋杆菌属等,观察组较对照组减少的物种有丹毒丝菌科、链球菌属、真杆菌属、多尔氏菌属、肠杆菌属、别样棒菌属、假单胞菌属、寡氧单胞菌属、贪铜菌属等。结论:解黄酮菌属、变形菌属的增加及丹毒丝菌科的减少能够引起一系列的生物代谢过程异常从而诱发机体的免疫失调,进而影响TNBC的发生发展。假单胞菌属的减少、解黄酮菌属的增加则导致TNBC患者更易出现肝郁气滞相关症状。多尔氏菌属的减少则会削弱免疫疗法对TNBC的疗效。综上,宿主肠道菌群的失调不仅能够影响TNBC的发生、进展与治疗,同时在中医证候的形成与变化过程中也发挥重要作用。
Objective:To study the variety and difference of intestinal flora in patients with triple negative breast cancer(TNBC)of liver depression and Qi stagnation type.Methods:According to the inclusion and exclusion criteria,eight patients with TNBC of liver depression and Qi stagnation type were included as the observation group,and 10 healthy volunteers were included as the control group.Fecal samples were collected respectively.The abundance and distribution of intestinal flora in the two groups were detected by 16S rDNA sequencing technology,and the genuses with significant differences(P<0.05)between the two groups were found by difference analysis.Results:The species richness of intestinal flora in patients with TNBC and healthy people was significant,and there was a significant difference in the distribution of intestinal flora between the two groups(P<0.05).Flavonifractor,Proteus,Enhydrobacter and so on were increased in the observation group compared with the control group,and the species less than the control group included Erysipelotrichaceae,Streptococcus,Eubacterium and so on.Conclusion:The increase of Flavonifractor and Proteus and the decrease of Erysipelotrichaceae can cause a series of abnormal biological metabolic processes to induce immune disorder,which in turn affects the occurrence and development of TNBC.The decrease of Pseudomonas and the increase of Flavonifractor lead to the symptoms of liver depression and Qi stagnation in TNBC patients.The reduction of Dorea will weaken the efficacy of immunotherapy on TNBC.In summary,the imbalance of host intestinal flora can not only affect the occurrence,progress,and treatment of TNBC,but also play an important role in the formation and change of traditional Chinese medicine syndromes.
出处
《中医临床研究》
2024年第35期1-6,共6页
Clinical Journal Of Chinese Medicine
基金
浙江省中医药科技计划青年人才基金项目(2022ZQ015)。
关键词
三阴性乳腺癌
肝郁气滞
中医证型
肠道菌群
Triple negative breast cancer
Liver depression and Qi stagnation
Traditional Chinese medicine syndrome
Intestinal flora
作者简介
孙梦莹(1999-),女,浙江宁波人,在读硕士研究生,研究方向为中医药防治肿瘤疾病的研究;刘远财,在读硕士研究生,研究方向为中医药防治肿瘤疾病的研究;张爱琴,女,主任中医师,博士,研究方向为中医药防治肿瘤疾病的研究;通讯作者:陈卓(1994-),浙江杭州人,住院医师,硕士,研究方向为中医药防治肿瘤疾病的研究。
