摘要
目的 探索瘀毒内阻证、气血两虚证结直肠癌患者肠道菌群结构的差异,为该类患者的治疗提供参考。方法 收集原发性结直肠癌患者的粪便标本,根据中医辨证分型分为瘀毒内阻证组(SPOS组)和气血两虚证组(QBDS组),其中SPOS组患者17例,QBDS组患者13例。使用16S rRNA基因扩增测序法鉴定2组患者肠道菌群的多样性和丰度,并分析组间物种差异。结果SPOS组患者肠道菌群Chao1指数(414.08±52.21vs803.07±294.53, P<0.001)、 Ace指数(419.84±52.83vs830.28±310.28, P<0.001)均低于QBDS组。组间差异分析显示组内差异大于组间差异(Anosim,R=0.138 7,P=0.017)。2组患者肠道菌群主要为厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidota)、变形菌门(Proteobacteria)、放线菌门(Actinobacteriota)和疣微菌门(Verrucomicrobiota)。LEfSe分析显示SPOS组有7个生物标志物菌群,在属水平上为栖粪杆菌属(Faecalibacterium)、普雷沃菌属(Prevotella)、瘤胃球菌属(Ruminococcus);QBDS组有10个生物标志物菌群,在种、属水平上为阿克曼菌属(Akkermansia),嗜黏蛋白阿克曼菌(Akkermansia_muciniphila),差异均具有统计学意义(均P<0.05)。结论 结直肠癌瘀毒内阻证、气血两虚证患者的肠道菌群多样性和丰度存在显著差异,其差异可能有助于区分临床中医证型。
Objective To observe the differences in structure of intestinal flora between colorectal cancer(CRC)patients with sstasis and poison obstruction syndrome(SPOS group)and those with Qi and blood deficiency syndrome(QBDS group),and provide a reference for the treatment.Methods Fecal samples of patients with primary CRC were collected and divided into SPOS group(n=17)or QBDS group(n=13)according to TCM differentiation classification.The diversity and abundance of fecal gut microbiota in the two groups were identified using 16S rRNA gene amplification sequencing,and the species differences between groups were analyzed.Results The Chao1 index(414.08+52.21 vs 803.07±294.53,P<0.001)and Ace index(419.84±52.83 vs 830.28±310.28,P<0.001)in SPOS group were lower than in QBDS group respectively.The inter-group difference analysis showed that the intra-group difference was greater than the inter-group difference(Anosim,R=0.1387,P=0.017).At the level of phylum,Firmicutes,Bacteroidota,Proteobacteria,Actinobacteriota and Verrucomicrobiota were dominant in the two groups.LEfSe analysis showed that there were 7 biomarker flora in the SPOS group,which at the genus level were Faecalibacterium,Prevotella and Ruminococcus,while the QBDS group had 10 biomarker flora,which at the genus and species level were Akkermansia,and Akkermansia_muciniphila;the differences were statistically significant(all P<0.05).Conclusion There were obvious differences in the diversity and abundance of intestinal flora between the SPOS group and QBDS group of colorectal cancer,which may help distinguishclinical TCM syndromes.
作者
归明彬
王雅楠
邹敏
涂浩
杨自杰
高峰
GUI Mingbin;WANG Yanan;ZOU Min;TU Hao;YANG Zijie;GAO Feng(Department of Colorectal&Anal Surgery,the 94Oth Hospital of Joint Logistics Support Force of Chinese People's Liberation Army,Lanzhou,Gansu 730050,China;不详)
出处
《中国微生态学杂志》
CAS
CSCD
2023年第7期797-801,805,共6页
Chinese Journal of Microecology
基金
甘肃省重点研发项目(20YF8FA098)。
关键词
结直肠癌
肠道菌群
16S
rDNA
中医证型
辨证分型
Colorectal cancer
Intestinal flora
16S rDNA
TCM syndrome types
Syndrome differentiation
作者简介
归明彬(1994-),男,医学硕士,从事结直肠肿瘤与基础研究,E-mail:guimingbin@163.com;通信作者:高峰,医学博士,主任医师,博士研究生导师,从事结直肠肿瘤与基础研究,E-mail:gaofeng994512163.com。
