摘要
轮作是应对药用植物三七连作障碍的有效途径,而不同轮作模式对土壤真菌群落的调节效应还鲜见报道.研究利用高通量测序技术对轮作滇重楼和滇黄精两种药用植物根际土壤真菌群落进行了定量分析,对理化性质与酶活性进行了检测,并与新地和连作地三七根际特征进行了比较,结果表明:三七连作导致根际土壤真菌多样性显著降低,癣囊腔菌属(Plectosphaerella)等潜在病原菌群相对丰度大幅升高,可能是连作障碍的主要成因.与连作三七相比较,轮作滇重楼和滇黄精根际真菌多样性显著升高,癣囊腔菌属相对丰度分别降低27.76%和33.33%,而潜在有益菌群木霉属(Trichoderma)相对丰度分别升高433.06%和2 353.32%;此外,非度量多维尺度分析(NMDS)表明,轮作滇黄精根际土壤真菌群落特征较非连作三七更为相似,且与连作三七根际土壤相比,磷酸酶与蔗糖酶活性显著增加(P<0.05);轮作滇重楼与滇黄精生长状况良好,受三七种植的影响较小.因此,轮作滇重楼与滇黄精对三七连作土壤真菌群落均具有良好的调节效应,有望成为应对三七连作障碍、缩短轮作年限的有力措施.
Rotation is an effective way to cope with the continuous cropping obstacle(CCO)of medicinal plant Panax notoginseng(PN),however,the regulatory effects of different rotation modes on soil fungal communities are rarely reported.Here we analyzed the fungal communities in the rhizosphere soil of Paris polyphylla var.yunnanensis(PP)and Polygonatum kingianum(PK)after P.notoginseng cultivation using high-throughput sequencing technology;moreover,soil physicochemical properties and enzyme activities were tested,and compared with the rhizosphere soil of PN growing in new soil and continuous cropping soil.The results showed that continuous cropping of PN resulted in a significant decrease in fungal diversity in the rhizosphere soil,and a significant increase of the relative abundance(RA)of potential pathogenic Plectosphaerella,which may lead to the CCO.Compared with PN under continuous cropping,fungal diversity in the rhizosphere of PP and PK was significantly increased,and the RA of Plectosphaerella decreased by 27.76%and 33.33%,respectively,however,the RA of potential beneficial Trichoderma increased by 433.06%and 2353.32%,respectively;moreover,non-metric multidimensional scaling(NMDS)analysis indicated that soil fungal community in the rhizosphere of PK was more similar to the non-continuous cropping soil of PN,and phosphatase and sucrase activities in the rhizosphere of PK were significantly increased comparing to continuous cropping of PN.PP and PK show normal growth,which are less affected by the PN cultivation.Therefore,the rotation of PP and PK has a good regulatory effect on the soil fungal community under continuous cropping of PN,which is expected to become an effective measure to cope with the CCO of PN by shortening the rotation period,moreover,the improvement effect of PK rotation could be more effective comparing to the rotation of PP.
作者
彭佳
官会林
王豪吉
魏富刚
杨绍周
李相川
王勇
徐武美
PENG Jia;GUAN Huilin;WANG Haoji;WEI Fugang;YANG Shaozhou;LI Xiangchuan;WANG Yong;XU Wumei(Yunnan Provincial Observation and Research Station of Soil Degradation and Restoration for Cultivating Plateau Traditional Chinese Medicinal Plants,Yunnan Normal University,Kunming 650500,China;Wenshan Miaoxiang Sanqi Technology Co.,Ltd.,Wenshan 663099,China;Wenshan Sanqi Institute of Science and Technology,Wenshan University,Wenshan 663099,China)
出处
《云南师范大学学报（自然科学版）》
2024年第2期61-68,共8页
Journal of Yunnan Normal University:Natural Sciences Edition
基金
国家自然科学基金资助项目(31901126)
云南省“兴滇英才支持计划”青年人才资助项目(XDYC-QNRC-2022-0247)
云南省院士专家工作站资助项目(202305AF150027,YSZJGZZ-2021062)。
关键词
三七
连作障碍
轮作
真菌群落
Panax notoginseng
Continuous cropping obstacle
Crop rotation
Soil fungal community
作者简介
彭佳(1999-),女,江西吉安人,硕士研究生,主要从事药用植物栽培方面研究;通信作者:徐武美.E-mail:xuwumei@ynnu.edu.cn.
