摘要
艾叶为临床常用中药材,岭南红脚艾基原南艾蒿与艾同为菊科蒿属艾组植物,为岭南民间艾叶的地方习用品。该研究运用气相色谱-三重四极杆质谱(GC-MS)对27批岭南红脚艾及13批传统艾叶样品挥发油成分进行检测,分析二者挥发性成分种类的异同,采用内标法结合多反应监测模式(MRM),对6种主要挥发性成分含量进行测定,并对测定结果进行层次聚类分析(HCA)和正交偏最小二乘法判别分析(OPLS-DA)。结果表明,艾叶挥发油平均含量高于红脚艾,且挥发油成分的种类更为丰富,多为沸点较低的组分,而红脚艾中多为沸点较高的组分。红脚艾和艾叶中挥发性成分均主要为萜类化合物,艾叶中主要含有单萜类化合物,红脚艾中则主要含有倍半萜类化合物,同时艾叶中的含氧衍生物含量明显高于红脚艾。化学计量学方法分析表明,HCA和OPLS-DA均能将红脚艾和艾叶区分开,二者挥发性成分差异显著。该研究可为红脚艾药材的质量评价提供科学依据,同时也为红脚艾作为岭南地方习用药材的合理性提供数据支持。
Artemisiae Argyi Folium is commonly used in clinical practice.Artemisiae Verlotori Folium,the dried leaves of Artemisia verlotorum,is often used as a folk substitute for Artemisiae Argyi Folium in Lingnan area.In this study,gas chromatographytriple quadrupole mass spectrometry(GC-MS)was used to detect the volatile oil components of 27 samples of Artemisiae Verlotori Folium and 13 samples of Artemisiae Argyi Folium,and the volatile components were compared between the two species.The internal standard method was combined with multi-reaction monitoring mode(MRM)to determine the content of six major volatile components.Hierarchical clustering analysis(HCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA)were carried out for the content data.The results showed that the Artemisiae Argyi Folium samples had higher content and more abundant volatile oils than the Artemisiae Verlotori Folium samples.Artemisiae Argyi Folium mainly had the components with lower boiling points,while Artemisiae Verlotori Folium mainly had the components with higher boiling points.Terpenoids were the main volatile components in Artemisiae Verlotori Folium(mainly sesquiterpenoids)and Artemisiae Argyi Folium(monoterpenoids).In addition,Artemisiae Argyi Folium had higher content of oxygen-containing derivatives than Artemisiae Verlotori Folium.Furthermore,the stoichiometric analysis showed that the two species could be distinguished by both HCA and OPLS-DA,indicating that the volatile components of the two were significantly different.This study can provide a scientific basis for the quality evaluation and data support for the local rational application of Artemisiae Verlotori Folium in Lingnan.
作者
邓静
吴婷芬
钟楚楚
马志国
曹晖
林泽斌
张英
吴孟华
DENG Jing;WU Ting-fen;ZHONG Chu-chu;MA Zhi-guo;CAO Hui;LIN Ze-bin;ZHANG Ying;WU Meng-hua(Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Informatization,Lingnan Resources Branch of National Engineering Research Center for Modernization of Traditional Chinese Medicine,Research Center for Traditional Chinese Medicine of Lingnan(Southern China),College of Pharmacy,Jinan University,Guangzhou 510632,China;Cao Hui′s Studio for Inheritance of National Famous Traditional Chinese Medicine Experts,Guangzhou 510632,China;Cao Hui′Expert Workstation in Yunnan Province,Kunming 655211,China;Guangdong Geeching Biomedical Technology Co.,Ltd.,/Healthcare Resource Branch of National Engineering Research Center for Modernization of Traditional Chinese Medicine,Huizhou 516081,China)
出处
《中国中药杂志》
CSCD
北大核心
2023年第23期6334-6346,共13页
China Journal of Chinese Materia Medica
基金
广东省重点领域研发计划项目(2020B1111110005)
国家重点研发计划项目(2019YFC1711500)
广东省基础与应用基础研究基金项目(2020A1515010577)
全国名老中医药专家传承工作室项目(国中医药人教函〔2022〕75号)
云南省曹晖专家工作站项目(202005AF150011)
全国中药特色传承人才项目(T20234832002)。
关键词
红脚艾
艾叶
GC-MS
层次聚类分析(HCA)
正交偏最小二乘法判别分析(OPLS-DA)
Artemisiae Verlotori Folium
Artemisiae Argyi Folium
GC-MS
hierarchical clustering analysis(HCA)
orthogonal partial least squares-discriminant analysis(OPLS-DA)
作者简介
通信作者:张英,副教授,硕士生导师,研究方向为中药资源、中药炮制、中药材及饮片质量评价,E-mail:carolynzy@163.com;通信作者:吴孟华,副教授,硕士生导师,研究方向为岭南传统中药的质量标准与岭南特色中药炮制,E-mail:zyfxwmh@163.com;邓静,硕士研究生,研究方向为中药质量控制,E-mail:1243909611@qq.com。
