摘要
采用UPLC-LTQ-Orbitrap-MS鉴定蕲艾中的酚酸和黄酮类成分,UPLC-DAD测定药材中主要成分的含量。采用Xtimate UHPLC C18色谱柱(2. 1 mm×100 mm,1. 8μm),以乙腈-0. 1%甲酸水溶液梯度洗脱,流速0. 4 m L·min-1,检测波长330nm,质谱扫描为正离子模式。通过质谱数据解析、对照品和文献对照,共鉴定、推导出蕲艾中6个酚酸和10个黄酮类成分,并建立了新绿原酸、绿原酸、隐绿原酸、异绿原酸A、异绿原酸B、异绿原酸C、茵陈色原酮、高车前素、棕矢车菊素、5,7,3'-三羟基-6,4',5'-三甲氧基黄酮、异泽兰黄素和蔓荆子黄素12个成分的含量测定方法,各成分质量浓度与峰面积具有较好的线性关系;精密度、重复性和稳定性良好(RSD<3. 0%);加样回收率为103. 3～109. 2%。该方法新颖、快速、准确,可作为蕲艾全面可靠的质量控制方法,应用于不同产地艾叶中相关成分的测定,发现蕲艾中的成分含量具有独特性。
The study aims to qualitatively and quantitatively analyze phenolic acids and flavonoids in Artemisiae Argyi Folium cultivated in Qichun(Qiai) for the quality control of this genuine regional herbs. UPLC-LTQ-Orbitrap-MS was used for rapid separation and structural identification of the constituents. Samples were separated on an UPLC column(2. 1 mm×100 mm,1. 8 μm) by gradient elution using 0. 1% formic acid and acetonitrile as mobile phases at a flow rate of 0. 4 m L·min^-1. By UPLC-LTQ-Orbitrap-MS,16 compounds including phenolic acids and flavonoids were identified by comparison with reference standards or literature data. For quantitative analysis,12 identified compounds were simultaneously determined by UPLC-DAD at wavelengths of 330 nm. The method was validated with respect to linearity,precision,repeatability,stability and recovery. The contents of these compounds were found to differ significantly between the samples from Qichun and other areas. This strategy was novel,effective and straightforward,which provided a potential approach for holistic quality control of Qiai.
作者
王小俊
邓玉环
张丽萍
郑国华
陈林霖
方颖
WANG Xiao-jun;DENG Yu-huan;ZHANG Li-ping;ZHENG Guo-hua;CHEN Lin-lin;FANG Ying(Key Laboratory of Traditional Chinese Medicine Resource and Compound Prescription,Ministry of Education,Hubei University of Chinese Medicine,Wuhan 430065,China;Lishizhen Pharmaceutical Group Co. ,Ltd.,Hubei Engineering Research Center for Application of Traditional Chinese Medicine Resources ,Hiianggang 435300,China;School of Pharmacy,Hubei University of Chinese Medicine,Wuhan 430065,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2019年第5期983-989,共7页
China Journal of Chinese Materia Medica
基金
湖北省教育厅科学技术研究项目(D20182005)
关键词
液相色谱-质谱
蕲艾
酚酸
黄酮
UPLC-MS
Artemisiae Argyi Folium
phenolic acids
flavonoids
作者简介
王小俊,硕士研究生,主要从事中药药效物质基础研究,Tel:(027)68890247,E-mail:18064120377@163.com;通信作者:陈林霖,Tel:(027)68890247,E-mail:lin-lin.chen@hotmail.com;通信作者:方颖,Tel:(027)68897069,E-mail:fangying@hbtcm.edu.cn.
