摘要
目的:研究钟花龙胆Gentiana nanobella干燥全株的化学成分。方法:采用硅胶柱色谱和凝胶柱色谱等对钟花龙胆干燥全株75%乙醇提取物乙酸乙酯萃取部位进行分离纯化,得到的单体化合物通过理化性质及现代波谱技术进行结构鉴定。结果:从钟花龙胆干燥全株75%乙醇提取物乙酸乙酯萃取部位分离得到8个单体化合物,分别鉴定为:熊果醇(1)、乌苏酸(2)、1β,2α,3α,24-四羟基-12-烯-28-乌苏酸(3)、山楂酸(4)、钩藤苷元C(5)、2α,3α,24-三羟基-12-烯-28-齐墩果酸(6)、胡萝卜苷(7)、(7'Z,10'Z,13'Z)-十六碳三烯酸单甘油酯(8)。结论:所有化合物均首次从钟花龙胆中分离得到,其中钩藤苷元C(5)、(7'Z,10'Z,13'Z)-十六碳三烯酸单甘油酯(8)为首次从龙胆属植物中分离得到。
Objective:To study the chemical constituents of the dried whole-plant of Gentiana nanobella.Methods:Silica gel column chromatography and gel permeation chromatography were used to isolate and purify the ethyl acetate fraction of the 75%ethanol extract of the dried whole-plant of G.nanobella.The isolated compounds were identified by physicochemical properties and modern spectroscopic techniques.Results:Eight compounds were isolated from the ethyl acetate fraction of the 75%ethanol extract of the dried whole-plant of G.nanobella and identified as uvaol(1),ursolic acid(2),1β,2α,3α,24-tetrahydroxyurs-12-en-28-oic acid(3),maslinic acid(4),uncargenin C(5),2α,3α,24-trihydrxyolean-12-en-28-oic acid(6),daucosterol(7),and(7'Z,10'Z,13'Z)-hexadecatrienoic acid monoglyceride(8).Conclusion:All the compounds were isolated from G.nanobella for the first time,among which uncargenin C(5)and(7'Z,10'Z,13'Z)-hexadecatrienoic acid monoglyceride(8)were first isolated from genus Gentiana.
作者
凌浩威
李靖
史凯旋
刘蓉
肖朝江
姜北
Ling Haowei;Li Jing;Shi Kaixuan;Liu Rong;Xiao Chaojiang;Jiang Bei(Yunnan Key Laboratory of Screening and Research on Anti-Pathogenic Plant Resources from Western Yunnan,Dali,Yunnan 671000,China;College of Pharmacy,Dali University,Dali,Yunnan 671000,China)
出处
《大理大学学报》
2023年第8期6-10,共5页
Journal of Dali University
基金
国家自然科学基金项目(81960637)
云南省滇西抗病原植物资源筛选研究重点实验室项目(202105AG070003)。
关键词
钟花龙胆
化学成分
提取分离
三萜
Gentiana nanobella
chemical constituents
extraction and separation
triterpenoids
作者简介
第一作者:凌浩威,硕士研究生,主要从事天然产物研究;通信作者:姜北,教授,博士,E-mail:jiangbei@dali.edu.cn。
