摘要
目的:研究绒毛灯笼花Agapetes lacei var.tomentella干燥块根中的化学成分。方法:采用硅胶柱色谱和薄层色谱法对绒毛灯笼花块根75%乙醇提取物乙酸乙酯部位进行分离纯化,依据理化性质及现代波谱技术对化合物进行结构鉴定。结果:从绒毛灯笼花块根75%乙醇提取物乙酸乙酯部位中分离得到10个化合物,分别鉴定为:3α,23-O-异亚丙基-2α,19α-二羟基-12-烯-28-乌苏酸(1)、3β,23-O-异亚丙基-2α,3β,23-三羟基-12-烯-28-齐墩果酸(2)、2α,3α,19α,23-四羟基-12-烯-28-乌苏酸(3)、2α,3β,23-三羟基-12-烯-28-齐墩果酸(4)、3β-乙酰乌苏酸(5)、乌苏酸(6)、β-谷甾醇(7)、5α,6β-二羟基谷甾醇(8)、胡萝卜苷(9)、4-羟基丁酸(10)。结论:所有化合物均为首次从绒毛灯笼花中分离得到,除化合物7和9外,其余化合物均为首次从树萝卜属植物中分离得到。
Objective:To study the chemical constituents of the dried root of Agapetes lacei var.tomentella.Methods:Silica gel column chromatography and thin layer chromatography were used to isolate and purify the ethyl acetate fraction of the 75%ethanol extract of the root of A.lacei var.tomentella,and the chemical structures of the compounds were identified based on physicochemical properties and modern spectroscopic techniques.Results:Ten compounds were isolated from the ethyl acetate fraction of the 75%ethanol extract of the root of A.lacei var.tomentella and identified as 3α,23-O-isopropylidenyl-2α,19α-dihydroxyurs-12-en-28-oic acid(1),3β,23-O-isopropylidenyl-2α,3β,23-trihydroxyolean-12-en-28-oic acid(2),2α,3α,19α,23-tetrahydroxyurs-12-en-28-oic acid(3),2α,3β,23-trihydroxyolean-12-en-28-oic acid(4),3β-acetoxy ursolic acid(5),ursolic acid(6),β-sitosterol(7),5α,6β-dihydroxysitosterol(8),daucosterol(9),and 4-hydroxybutanoic acid(10).Conclusion:All the compounds were isolated from A.lacei var.tomentella for the first time,except for compounds 7 and 9,the other compounds were first isolated from genus Agapetes.
作者
李靖
凌浩威
史凯旋
刘蓉
肖朝江
姜北
Li Jing;Ling Haowei;Shi Kaixuan;Liu Rong;Xiao Chaojiang;Jiang Bei(Yunnan Key Laboratory of Screening and Research on Anti-Pathogenic Plant Resources from Western Yunnan,Dali,Yunnan 671000,China;College of Pharmacy,Dali University,Dali,Yunnan 671000,China)
出处
《大理大学学报》
2023年第8期1-5,共5页
Journal of Dali University
基金
国家自然科学基金项目(81960637)
云南省滇西抗病原植物资源筛选研究重点实验室项目(202105AG070003)
云南省教育厅科学研究基金项目(2022Y822)。
关键词
绒毛灯笼花
树萝卜属
化学成分
三萜
甾体
Agapetes lacei var.tomentella
Agapetes
chemical constituents
triterpenoids
steroids
作者简介
第一作者:李靖,硕士研究生,主要从事天然产物研究;通信作者:姜北,教授,博士,E-mail:jiangbei@dali.edu.cn。
