摘要
目的 探讨银翘散治疗亚急性甲状腺炎(SAT)的潜在作用机制。方法 借助中药药理学数据库获得银翘散中的主要成分及其作用靶点,通过UniProt数据库进行转化,在GeneCards、OMIM、DrugBank和PharmGKB数据库中检索SAT的靶基因,获取银翘散治疗SAT的潜在靶基因,绘制“成分-靶点-疾病”网络图,构建蛋白质-蛋白质相互作用网络,并进行基因本体论富集分析与京都基因与基因组百科全书通路分析。结果 银翘散中共有126个活性成分,其中主要成分为槲皮素、沃戈宁、柚皮素等。银翘散治疗SAT的潜在作用靶点共有134个,其中核心靶点为丝裂原激活蛋白激酶3、血管内皮生长因子A、低氧诱导因子-1α、蛋白激酶B1等。与SAT密切相关的通路有动脉粥样硬化、病毒感染、磷脂酰肌醇3激酶-蛋白激酶B信号通路、白细胞介素-17信号通路等。关键成分和核心靶点具备良好的组合活性。结论 银翘散中的活性成分可能就是通过抗炎、抗氧化、抗病毒等方式来达到治疗SAT的效果。
Objective To explore the potential mechanism of Yinqiao powder in the treatment of subacute thyroiditis(SAT).Methods The main components and their targets in Yinqiao powder were obtained by database of TCMSP,and the target genes of SAT were searched in GeneCards,OMIM,DrugBank and PharmGKB databases through transformation of UniProt database,so as to obtain the potential target genes of Yinqiao powder in the treatment of SAT.And a"component-target-disease"network diagram was drawn to construct a protein-protein interaction network,gene ontology enrichment analysis and Kyoto encyclopedia of genes and genomes pathway analysis were carried out.Results There were 126 active ingredients in Yinqiao powder,including quercetin,vogonin,naringenin,etc.There were 134 potential targets of Yinqiao powder in treating SAT,of which the core targets were mitogen activated protein kinase 3,vascular endothelial growth factor A,hypoxia inducible factor-1α,protein kinase B1,etc.The pathways closely related to SAT included atherosclerosis,virus infection,phosphatidylinositol 3-kinase protein kinase B signaling pathway,interleukin-17 signaling pathway,etc.The key components and core targets had good combinatorial activity.Conclusion The active ingredients in Yinqiao powder may achieve the therapeutic effect of SAT through anti-inflammatory,antioxidant,antiviral and other methods.
作者
章艳艳
陈言鹏
倪启超
ZHANG Yanyan;CHEN Yanpeng;NI Qichao(Department of Pathology,the Third People′s Hospital of Nantong City/the Third Affiliated Hospital of Nantong University,Nantong,Jiangsu 226006,China;Department of General Surgery,the Affiliated Hospital of Nantong University,Nantong,Jiangsu 226001,China)
出处
《现代医药卫生》
2023年第13期2189-2198,共10页
Journal of Modern Medicine & Health
关键词
银翘散
亚急性甲状腺炎
网络药理学
靶基因
Yinqiao powder
Subacute thyroiditis
Network pharmacology
Target gene
作者简介
章艳艳(1997-),本科,初级病理技师,主要从事甲状腺疾病病理研究;通信作者:倪启超,E-mail:nqcuser@163.com。
