摘要
为探究甜高粱幼苗对干旱胁迫的响应机制和对干旱胁迫的忍受范围,通过盆栽试验的方式,设置土壤相对含水量65%~70%(CK)、55%~60%(T1)、40%~45%(T2)、30%~35%(T3)和20%~25%(T4)5个水平,研究干旱胁迫对甜高粱叶片光合色素含量、保护酶活性及活性氧代谢的影响。结果表明,随着干旱胁迫强度的增强,甜高粱幼苗叶片叶绿素a、叶绿素b、叶绿素a+b和类胡萝卜素均表现出降低趋势,但T1处理与对照差异均不显著(P>0.05),且在T4处理下值最低,较对照分别降低54.31%,44.44%,52.65%,51.47%,与对照差异极显著(P<0.01)。随着干旱胁迫强度的增加,甜高粱幼苗叶片保护酶SOD、CAT、POD、APX活性呈先升高再降低的单峰变化趋势,但SOD、POD、APX活性在T1处理下值最大,较对照分别增加7.45%,13.94%,24.63%,CAT在T2处理下值最大,较对照增加3.48%。甜高粱幼苗叶片超氧阴离子随干旱胁迫强度的增加而升高,但在轻度胁迫下(T1)与对照差异不显著(P<0.05);4个处理的甜高粱幼苗叶片过氧化氢含量随干旱胁迫强度的增加而升高,且与对照差异均显著(P<0.05)。可见,当土壤相对含水量不低于55%~60%时,甜高粱幼苗保护酶活性高于对照,且光合色素、超氧阴离子含量与对照差异不显著(P>0.05)。
In order to explore the response mechanism and tolerance range of sweet sorghum seedlings to drought stress,the effects of drought stress on photosynthetic pigment content,protective enzyme activity and active oxygen metabolism of sweet sorghum leaves were studied by setting five levels of soil relative water content:65%-70%(CK),55%-60%(T1),40%-45%(T2),30%-35(T3)and 20%-25%(T4).The results showed that with the increase of drought stress intensity,chlorophyll a,chlorophyll b,chlorophyll a+b and carotenoid in sweet sorghum seedlings showed a decreasing trend,but the T1 treatment compared with control was not significant,and the value was the lowest under T4 treatment,which were decreased by 54.31%,44.44%,52.65%and 51.47%than that of control,respectively(P<0.01).With the increase of drought stress intensity,the activities of SOD,cat,pod and APX in leaves of sweet sorghum seedlings increased first and then decreased.Among them,the activities of SOD,POD and APX were the largest under T1 treatment,and increased by 7.45%,13.94%and 24.63%respectively compared with the control.The activity of CAT were the largest under T2 treatment,and increased by 3.48%compared with the control.The superoxide anion in leaves of sweet sorghum seedlings increased with the increasing of drought stress intensity,but there was no significant difference between mild stress(T1)and the control.With the increasing of drought stress intensity,the hydrogen peroxide content in the leaves of sweet sorghum seedlings increased,and there was significant difference between the four treatments and the control(P<0.05).It can be seen that when the soil relative water content is not less than 55%-60%,the protective enzyme activity of sweet sorghum seedlings is higher than that of the control,and the contents of photosynthetic pigment and superoxide anion are not significantly different from the control(P>0.05).
作者
陈敏菊
孟彦
孟宪政
CHEN Minju;MENG Yan;MENG Xianzheng(Shangqiu Academy of agriculture and Forestry Sciences,Shangqiu,Henan 476000,China)
出处
《天津农业科学》
CAS
2021年第9期1-4,共4页
Tianjin Agricultural Sciences
基金
河南省四优四化项目(20190411)。
关键词
甜高粱
干旱胁迫
光合色素
保护酶
活性氧
sweet sorghum
drought stress
photosynthetic pigment
protective enzyme
active oxygen
作者简介
陈敏菊(1981—),女,河南商丘人,助理研究员,主要从事高粱育种与生理栽培方面研究;通讯作者:孟彦(1978—),男,河南商丘人,副研究员,主要从事高粱育种与生理栽培方面研究。
