摘要
利用碳纤维电离质谱(CFI-MS)法直接对未经前处理的中药中挥发性成分进行快速分析,从桂枝、当归、川芎、广藿香油、复方川芎胶囊和四物颗粒中成功地表征多种成分,包括烃类、萜类、醛类、酮类、酚类、酯类、甾体类等。采用CFI-MS法快速分析不同新鲜程度的桂枝和橘皮(陈皮),通过质谱图可以鉴别其新鲜程度。该方法简便、可靠、快速,可以对中药中挥发性成分进行快速直接的系统表征,为药效物质基础及质量控制研究提供理论依据。
The carbon fiber ionization mass spectrometry(CFI-MS)method was used to quickly and directly analyse volatile components in traditional Chinese medicine(TCM)without pretreatment.Three key factors affecting the signal intensity of CFI-MS were optimized,which was voltage,horizontal distance between carbon fiber tip and mass spectrometer entrance,and vertical distance between TCM sample and carbon fiber tip.Taking cinnamaldehyde as an example,the signal intensity changes of CFI-MS on samples with different concentrations were investigated.And then the optimized CFI-MS was used to quickly and directly analyse TCMs without any pre-treatment,that was Ramulus Cinnamomi,Angelica sinensis,Ligusticum chuanxiong,Patchouli oil,Fufangchuanxiong capsules and Siwu granules,as the result various components were successfully characterized,such as hydrocarbons,terpenes,aldehydes,ketones,phenols,esters,and steroids.In addition,the optimized CFI-MS was used to quickly and directly analyse the Ramulus Cinnamomi and tangerine peel of different freshness,and the freshness could be identified from the differences in mass spectra.The method is simple,reliable and rapid analysis,which can perform rapid and direct system characterization research on volatile components in TCM.The use of CFI-MS provides scientific theoretical basis for the research on the substance base and quality control of TCM.In addition,the residual compounds on the carbon fiber can be quickly removed by flame combustion.As a result,there is no cross-contamination,so CFI-MS shows the potential for high-throughput analysis.Compared with other AI-MS,CFI-MS device is simple,easy to operate,less adjustment parameters,rapid and efficient,time-saving and labor-saving in direct analysis.
作者
张强
苏越
郭寅龙
ZHANG Qiang;SU Yue;GUO Yin-long(Department of Criminal Science and Technology,Henan Police College,Zhengzhou 450046,China;Center for Chinese Medicine Therapy and Systems Biology,Institute for Interdisciplinary Medicine Sciences,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;National Center for Organic Mass Spectrometry in Shanghai,Shanghai Institute of Organic Chemistry,Chinese Academy of Sciences,Shanghai 200032,China)
出处
《质谱学报》
EI
CAS
CSCD
北大核心
2021年第2期118-128,共11页
Journal of Chinese Mass Spectrometry Society
基金
上海市科委“科技创新行动计划”化学试剂与仪器方法领域项目(19142201400)
教育部人文社会科学研究青年基金项目“敞开式离子化质谱在物证类司法鉴定中的应用”(20YJC820062)资助。
关键词
碳纤维电离质谱(CFI-MS)
快速分析
中药
挥发性成分
carbon fiber ionization mass spectrometry(CFI-MS)
rapid analysis
traditional Chinese medicine
volatile components
作者简介
张强(1988-),男(汉族),河南人,讲师,从事药物和毒品毒物分析、文件检验研究。E-mail:qiangzh126@126.com;通信作者:苏越(1965-),女(汉族),河南人,研究员,从事中药分析研究。E-mail:suyue@shutcm.edu.cn;通信作者:郭寅龙(1962-),男(回族),河南人,研究员,从事有机质谱研究。E-mail:ylguo@sioc.ac.cn。
