摘要
【目的】通过不同种源麻花艽的光合特性比较,分析其光合机构的驯化适应能力,为优质种质资源的筛选与引种培育提供科学依据.【方法】源自青藏高原地区不同海拔的五类麻花艽类群,栽培于青海省湟中县多巴镇实验苗圃,经1 a引种栽培驯化后,比较分析了其叶片光合色素、叶绿素荧光和光合气体代谢参数.【结果】较高海拔碌曲类群的叶绿素a、叶绿素b和类胡萝卜素含量最高;低海拔互助与同仁类群的胡萝卜素、叶绿素b含量低于碌曲和达日类群,而叶绿素a/b则高于碌曲和达日类群.源自高海拔的类群,光合速率(P n)较高,而气孔导度(G s)和胞间CO 2浓度(C i)则相对较低;蒸腾速率(T r)除低海拔互助显著较高外,其余不同类群间无明显差异.叶绿素荧光测定表明,PSⅡ有效光化学效率(F v′/F m′)和实际光化学效率(ΦPSⅡ)在达日和碌曲类群略低,光化学猝灭系数(qP)在各个类群间几乎无差异,但非光化学猝灭系数(NPQ)则达日和河南类群显著高于同仁类群.叶绿素荧光的光响应曲线显示,qP随光强增高而降低,而NPQ随光强增高而增高,两者均为高海拔达日和碌曲类群较高;相对光合电子传递速率(ETR)随光强增大而增高,高光强呈光抑制现象,且达日、碌曲与河南驯化类群的ETR高于互助和同仁类群,也具有较高初始斜率α、ETR m和EK值.【结论】经过1 a的驯化适应,不同种源麻花艽类群的光合特性存在着差异,与其起源地海拔有密切关系,其中源自高海拔达日、碌曲和河南麻花艽类群的P n,C i和叶绿素荧光特征均呈现较高的适应性和光合潜力,在引种栽培中具有较大利用空间.
【Objective】The differences of photosynthetic apparatus adaptation characteristics among germplasm of Gentiana straminea Maxim.were analyzed during the flowering season after one-year acclimation,which would provide the theoretical basis for G.straminea germplasm selection and culture.【Method】Pigment content,chloropphyll fluorescence parameters and photosynthetic characteristics were studied on five transplanted G.straminea populations from different sites cultured in Duoba garden for a year.【Result】The contents of carotenoids,chlorophyll a and b were highest in the leaves of Luqu population.On the whole,content of carotenoids,chlorophyll b and Car/Chl on Huzhu and Tongren population were lower than that of the Dari and Luqu population from the higher altitudes,while Chl a/b was higher than the laters'.The net photosynthesis rate(P n)was tended to increase with the altitude enhanced,while the stomatal conductance(G s),intercellular CO 2(C i)concentration showed declination on higher altitude populations.Except the Huzhu population,the evaporation rate(T r)has no clear difference among rest populations.Chloropphyll fluorescence analyses showed the PSⅡmaximal and actual photochemical efficiency(F v/F m andΦPSⅡ)of Dari and Luqu population showed a bit reduction,while there was no significant difference among all the populations.Photochemical quenching coefficient(qP)was very close among the five populations while the non-photochemical quenching coefficient(NPQ)appeared significant higher in Dari and Luqu populations than that of Tongren one.The light response curve showed that qP appeared fall while NPQ showed rise as the light intensity raised.Moreover,qP and NPQ was inclined to increase as the altitude raised and high light intensity caused photoinhibition.Electron transfer reaction(ETR)showed higher in the populations from high altitudes.In addition,the high altitude population Dari,Luqu and Henan also had higherα,ETRm and EK value than those of Tongren and Huzhu.【Conclusion】The photosynthetic variation among the G.streaminea populations after one-year acclimation,which was probably related to the original location altitude.Population from higher altitude such as Dari,Luqu and Henan had advantage on P n,C i and chlorophyll fluorescence character and occupied stronger adaption ability and photosynthetic potentiality,which would be helpful for utilization of high quality germplasm culture of this species in the future.
作者
周党卫
师生波
王环
王亚艺
王久利
李松龄
陈世龙
沈建伟
程庭峰
李小娟
ZHOU Dang-wei;SHI Sheng-bo;WANG Huan;WANG Ya-yi;WANG Jiu-li;LI Song-ling;CHEN Shi-long;SHEN Jian-wei;CHENG Ting-feng;LI Xiao-juan(Key Laboratory of Adaptation and Evolution of Plateau Biota,Chinese Academy of Sciences,Xining 810008,China;Tibetan Medicine Center,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining 810008,China.;Key Laboratory of Qinghai Plant Molecular Breeding,Chinese Academy of Sciences,Xining 810008,China;College of Ecological Environment and Resources,Qinghai Nationalities University,Xining 810007,China;Institute of Soil and Fertilizer,Qinghai University,Xining 810008,China)
出处
《甘肃农业大学学报》
CAS
CSCD
北大核心
2020年第5期84-93,100,共11页
Journal of Gansu Agricultural University
基金
青海省科技基础研究计划项目(2017-ZJ-702
2019-ZJ-7016
2017-ZJ-Y20)
青海省自然科学基金项目(2019-ZJ-976Q)
青海省创新平台建设专项项目-青海省作物分子育种重点实验室(2017-ZJ-Y14).
关键词
麻花艽
种源
驯化适应
海拔
光合作用
叶绿素荧光
Gentiana straminea Maxim.
germplasm source
acclimation
photosynthesis
light response curve
作者简介
第一作者:周党卫,副研究员,硕士生导师,主要从事高山植物生物学研究.E-mail:dangweizhou@sina.com。
