摘要
以黄瓜品种‘津优1号’为试材,研究利用生物质炭改善邻苯二甲酸二(2-乙基己基)酯(diethyl hexyl phthalate,DEHP)污染土壤时,对黄瓜幼苗生理特性的影响。结果表明DEHP污染土壤施入生物质炭可以改善DEHP对细胞膜的伤害,施入1%和2%生物质炭对黄瓜幼苗细胞膜稳定性的影响优于0.5%生物质炭。生物质炭可以提高黄瓜叶片SOD活性和叶绿素a、叶绿素b和类胡萝卜素含量,降低叶片POD和APX活性,对CAT的影响以0.5%生物质炭最显著。施入生物质炭对叶绿素荧光参数Fv/Fm无显著影响,但0.5%和1%生物质炭可以显著改善DEHP污染对qp、NPQ和ΦPSII的影响。灰色关联度分析结果表明,生物质炭和DEHP都是从影响光合色素的合成开始,进而影响NPQ和抗氧化酶系统,最后引起细胞膜的稳定性和叶绿素荧光特性的改变。综合分析利用生物质炭改善DEHP污染土壤时,1%生物质炭的效果优于0.5%和2%生物质炭。同时也表明,农田施用生物质炭时必须评估其对作物的风险。
In the present study,the cucumber‘Jinyan No.1’as test material,we investigated the effects of biochar on physiological characteristics of cucumber seedlings in soils contaminated with diethyl hexyl phthalate(DEHP).The results showed that biochar could remit the damage of DEHP on cell membrane,and the effects of 1%and 2%biochar were better than that of 0.5%biochar.Biochar could improve the activity of SOD and contents of chlorophyll a,chlorophyll b and carotenoid,reduce activities of POD and APX in leaves.The most significant influence on CAT activity was 0.5%biochar.Treatment with biochar had no significant effects on chlorophyll fluorescence parameters Fv/Fm.While,0.5%and 1%biochar treatment can significantly relieve the effects of DEHP pollution on qp,NPQ andΦPSII.Grey correlation analysis indicated that biochar and DEHP firstly influenced the photosynthetic pigment synthesis,then affected NPQ and antioxidant enzyme system,finally caused changes of cell membrane stability and chlorophyll fluorescence.Comprehensive analysis showed the effects of 1%biochar treatment on cucumber seedlings in DEHP contaminated soil was better than 0.5%and 2%biochar.At the same time,risk assessment on crops was very necessary when biochar was applied to farmland.
作者
李贞霞
李瑞静
穆娟
杨志龙
孙帅
严云
王广印
LI Zhenxia;LI Ruijing;MU Juan;YANG Zhilong;SUN Shuai;YAN Yun;WANG Guangyin(College of Horticulture and Landscape,Henan Institute of Science and Technology,Xinxiang,Henan 453003,China;Henan Province Engineering Research Center of Horticultural Plant Resource Utilization and Germplasm Enhancement,Xinxiang,Henan 453003,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2020年第1期73-82,共10页
Plant Physiology Journal
基金
河南省大宗蔬菜产业技术体系建设项目(S2010-03-G06)
河南省科技厅基础与前沿项目(142300410140)
河南省大学生创新创业训练计划项目(201810467009).
作者简介
通讯作者:李贞霞(lizhenxia196@163.com)。
