摘要
【目的】研究野生青海茄参不同组织及不同生长期的营养品质及最佳采收时期。【方法】以野生青海茄参为试验材料,利用分光光度计、酶标仪、高效液相色谱法分别对其3个组织(叶片、果实、根)中的含水量、可溶性蛋白、可溶性糖、总酚、类黄酮等12个指标进行检测。【结果】青海茄参不同组织营养品质含量差异较大,蔗糖是青海茄参可溶性糖的主要成分。其中鲜叶可溶性蛋白含量最高,老叶总酚含量最高,且与其他组织差异显著(P<0.05)。果实成熟度Ⅳ淀粉、还原糖、类黄酮含量最高。果实成熟度Ⅰ到成熟度Ⅳ生长指标先增大后减小,果实成熟度Ⅲ最佳且可溶性蛋白、可溶性糖的含量最高;可溶性糖与蔗糖、还原糖、淀粉呈极显著正相关;蔗糖与还原糖、淀粉呈显著正相关,与游离氨基酸呈极显著正相关;总酚与游离氨基酸、可溶性糖、蔗糖、淀粉呈负相关;类黄酮与可溶性蛋白,游离氨基酸呈负相关。【结论】野生青海茄参的适宜采收期为果实成熟度Ⅲ。
【Objective】To study the nutritional quality and optimal harvest time of wild Qinghai Man-dragora caulescens in different tissues and different growth periods.【Method】Using wild Qinghai M.caule-scens as a test material,12 indexes such as water content,soluble protein,soluble sugar,total phenolics,and flavonoids in its three tissues(leaves,fruits at various stages of ripeness,and roots)were detected by spectrophotometer,microplate reader,and high performance liquid chromatography.【Result】The nutritional quality content of different tissues of Qinghai M.caulescens varies widely,and sucrose is the main component of the soluble sugar of Qinghai M.caulescens.Among them,the soluble protein content of fresh leaves was the highest,and the total phenol content of old leaves was the highest,and there were significant differences with other tissues(P<0.05).The content of starch,reducing sugar and flavonoids in fruit IV is the highest.The growth index from fruit I to IV first increased and then decreased,and the content of soluble protein and soluble sugar was the highest in fruit III.Soluble sugars were significantly positively correlated with sucrose,reducing sugars,and starch.Sucrose was significantly positively correlated with reducing sugars and starch,and very significantly positively correlated with free amino acids.Total phenolics were negatively correlated with free amino acids,soluble sugars,sucrose,and starch,and flavonoids were negatively correlated with soluble proteins and free amino acids.【Conclusion】The suitable harvest period of wild Qinghai M.caulescens is fruit ripeness III.
作者
代思雨
段红俊
殷光晶
李强锋
赵艳艳
DAI Siyu;DUAN Hongjun;YIN Guangjing;LI Qiangfeng;ZHAO Yanyan(College of Agriculture and Animal Husbandry,Qinghai University,Xining 810016,China;Qinghai Forest Seed and Seedling Station,Xining 810099,China)
出处
《甘肃农业大学学报》
CAS
CSCD
北大核心
2024年第1期284-291,303,共9页
Journal of Gansu Agricultural University
基金
国家自然科学基金项目(31860575)
青海省林业厅林木种苗总站国家长期科研基地监测项目(2130211)。
关键词
青海茄参
不同组织
营养品质
差异分析
相关性分析
Qinghai Mandragora caulescens
different organizations
nutritional quality
difference analysis
correlation analysis
作者简介
第一作者:代思雨,硕士研究生。E-mail:dsy985787603@163.com;通信作者:赵艳艳,副教授,主要从事蔬菜栽培及分子逆境研究。E-mail:yltfy600158@163.com。
