摘要
目的探讨ER和TGF-β1与宫腔粘连的相关性。方法行宫腔镜检查并确诊为宫腔粘连(IUA)的患者60例作为实验组,中度30例,重度30例;对照组45例,因不孕症行宫腔镜检查未发现宫腔粘连的患者。采用实时荧光定量PCR方法检测各组子宫内膜中雌激素受体(ER)mRNA水平及转化生长因子-β1(TGF-β1)mRNA的水平;采用免疫印迹法检测各组子宫内膜中ER蛋白及TGF-β1蛋白的水平。结果对照组、中度IUA组及重度IUA组中ERmRNA分别为(0.93±0.19)、(0.6±0.28)及(0.51±0.21),重度IUA组ERmRNA较对照组降低,且差异有统计学意义(P<0.05),中度IUA组ERmRNA较对照组降低,且差异有统计学意义(P<0.05),但重度IUA组ERmRNA与中度IUA组ERmRNA间差异无统计学意义(P>0.05);ER蛋白在对照组、中度IUA组及重度IUA组依次降低,分别为(0.87±0.16)、(0.64±0.32)及(0.51±0.14),且组间比较差异均具有统计学意义(P<0.05);TGF-β1mRNA在对照组、中度IUA组及重度IUA组依次增高,分别为(0.92±0.3)、(1.58±0.32)及(2.31±0.28),且组间比较差异均具有统计学意义(P<0.05);TGF-β1蛋白在对照组、中度IUA组及重度IUA组分别为(0.37±0.15)、(1.08±0.29)及(1.09±0.31),重度IUA组TGF-β1蛋白较对照组增高,且差异有统计学意义(P<0.05),中度IUA组TGF-β1蛋白较对照组增高,且差异有统计学意义(P<0.05),但重度IUA组TGF-β1蛋白与中度IUA组TGF-β1蛋白间差异无统计学意义(P>0.05);3组中ERmRNA与TGF-β1mRNA均呈负相关(r=-0.643,P<0.05),3组中ER蛋白与TGF-β1蛋白呈负相关(r=-0.478,P<0.05)。结论ER及TGF-β1表达异常可能是宫腔粘连发生发展的因素。
Objective To discuss the correlation among Estrogen receptor(ER),Transforming growth factor-β1(TGF-β1)and intrauterine adhesion(IUA).Methods Sixty cases of patients diagnosed with IUA by hysteroscopy were colleceted as the experimental group,30 moderate cases and 30 severe cases.45 patients diagnosed as non-IUA by hysteroscopy due to infertility were treated as the control group.The levels of ER mRNA and TGF-β1mRNA in the endometrium of each group was detected by quantitative real-time polymerase chain reaction.The levels of ER protein and TGF-β1 protein in the endometrium of each group was detected by Western bloting.Results Compared with the control group,the levels of ERmRNA in the severe and moderate IUA groups were lower,there were significant differences(P<0.05).The level of ER protein in the endometrium was decreased in the moderate and severe groups(P<0.05).The level of TGF-β1mRNA in the endometrium were increased in the moderate and severe groups(P<0.05).Compared with the control group,the expression of TGF-β1 protein was higher in the severe and moderate IUA groups(P<0.05).The level of ERmRNA was negatively correlated with TGF-β1mRNA in the three group(r=-0.643,P<0.05).The expression of ER protein was negatively correlated with TGF-β1 protein in the three group(r=-0.478,P<0.05).Conclusion The abnormal expression of ER and TGF-β1 may be a factor for the occurrence and development of intrauterine adhesions.
作者
杜娟
康卉娴
DU Juan;KANG Huixian(Reproductive Medicine Centre,Yinchuan Women and Children Health Care Hospital,Yinchuan 750001,China)
出处
《宁夏医学杂志》
CAS
2020年第7期628-630,共3页
Ningxia Medical Journal
