摘要
目的研究红蓝花酒对大鼠宫腔粘连的改善作用及TGF-β1/Smads通路的调节作用。方法75只大鼠随机分为造模组(63只)及正常对照组(12只),采用刮勺搔刮法建立宫腔粘连大鼠模型,随机取3只大鼠进行造模验证后,分为宫腔粘连模型组、红蓝花酒低、中、高剂量组及阳性对照组,12只/组。红蓝花酒各剂量组分别灌胃给予大鼠0.5 g·kg^(-1)、1 g·kg^(-1)、2 g·kg^(-1)的红蓝花酒,阳性对照组给予大鼠1.028 mg·kg^(-1)的戊酸雌二醇,宫腔粘连模型组及正常对照组给予大鼠等量生理盐水,每天1次,连续给药24天。采用酶联免疫吸附法(ELISA)法检测大鼠血清及子宫组织肿瘤坏死因子(TNF)-α、白介素(IL)-1β、IL-6水平,苏木精-伊红(HE)染色法检测大鼠子宫组织病理学变化,并测量子宫内膜厚度,计算子宫内膜腺体数量,实时定量聚合酶链式反应(PCR)检测子宫组织TGF-β1、Smad2及Smad3 mRNA水平,免疫印迹法(Western blot)检测大鼠子宫组织TGF-β1、Smad2及Smad3蛋白水平。结果宫腔粘连模型组血清及子宫组织TNF-α、IL-1β、IL-6水平,子宫内膜厚度,子宫组织TGF-β1、Smad2及Smad3 mRNA及蛋白水平较正常对照组均显著升高(P<0.05),子宫内膜腺体显著减少(P<0.05);红蓝花酒各剂量组及阳性对照组血清及子宫组织TNF-α、IL-1β、IL-6水平,子宫内膜厚度,子宫组织TGF-β1、Smad2及Smad3 mRNA及蛋白水平较宫腔粘连模型组显著降低(P<0.05),大鼠子宫内膜腺体数量显著增加(P<0.05),且各项指标变化与红蓝花酒的剂量表现出依赖性。结论红蓝花酒能够修复大鼠宫腔粘连,抑制大鼠体内及子宫组织炎症水平,进而抑制大鼠子宫内膜的纤维化的发生及进展,其机制可能与调节TGF-β1/Smads通路有关。
Objective To study the effect of Honglanhua wine on the improvement of intrauterine adhesions in rats and the regulation of TGF-β1/Smads pathway.Methods 75 rats were randomly divided into model building group(63 rats)and normal control group(12 rats).A rat model of intrauterine adhesions was established by scraping with a spatula,and 3 rats were randomly selected for model verification.The rest of rats were randomly divided into uterine adhesion model group,honglanhua wine low,medium and high dose groups and positive control group,with 12 rats per group.Rats in the low,medium and high doses of honglanhua wine were given 0.5 g·kg^(-1),1 g·kg^(-1),2 g·kg^(-1) honglanhua wine by gavage respectively,while the positive control group was given Estradiol valerate of 1.028 mg·kg^(-1) by gavage,intrauterine adhesion model group and normal control group were given the same amount of normal saline,once a day for 24 days.Enzyme-linked immunosorbent assay(ELISA)method was used to detect rat serum and uterine tissue TNF-α,IL-1β,IL-6 levels,hematoxylin-eosin(HE)staining method was used to detect rat uterine histopathological changes,and measure the endometrial thickness,calculate the number of endometrial glands.Real-time quantitative polymerase chain reaction(PCR)was used to detect the levels of TGF-β1,Smad2 and Smad3 mRNA in the endometrial tissue,and Western blot was used to detect rats Uterine tissue TGF-β1,Smad2 and Smad3 protein levels.Results Compared with the normal control group,the uterine adhesion model group rat serum and endometrial tissue TNF-α,IL-1β,IL-6 levels,endometrial thickness,endometrial tissue TGF-β1,Smad2 and Smad3 mRNA and protein levels were significantly increased(P<0.05),and the number of endometrial glands in rats was significantly reduced(P<0.05);compared with the uterine adhesion model group,the serum levels and endometrial tissue TNF-α,IL-1β,IL-6 levels of the rats in the honglanhua wine groups and the positive control group,endometrial thickness,endometrial tissue TGF-β,Smad2 and Smad3 mRNA and protein levels were significantly reduced(P<0.05),the number of rat endometrial glands was increased significantly(P<0.05),and the changes of various indicators were dependent on the dose of Safflower wine.Conclusion Safflower wine repaired the state of intrauterine adhesions in rats,and inhibited the level of inflammation of rats with intrauterine adhesions,and thereby inhibited endometrial fibrosis in rats.The mechanism may be related to the regulation of the TGF-β1/Smads pathway.
作者
黄琼
吴媛媛
赵军玲
张胜男
王东芳
罗志娟
Huang Qiong;Wu Yuanyuan;Zhao Junling;Zhang Shengnan;Wang Dongfang;Luo Zhijuan(Ruikang Hospital Affiliated to Guangxi University of Traditional Chinese Medicine,Nanning 530011,China)
出处
《世界科学技术-中医药现代化》
CSCD
北大核心
2023年第3期1155-1161,共7页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
广西壮族自治区中医药管理局自筹经费科研课题(GZZC2020118):红蓝花酒用于预防人流术后宫腔粘连的临床研究,负责人:黄琼
广西壮族自治区中医药民族医药事业传承与发展专项资金项目([2022]2):罗志娟广西名中医传承工作室建设项目,负责人:罗志娟
作者简介
通讯作者:罗志娟,主任医师,主要研究方向:不孕不育、生殖内分泌及妇科炎症的临床与科研。
