摘要
建立了分散固相萃取-超高效液相色谱-串联线型离子阱(DSPE-UHPLC-QTRAP-MS/MS)测定鸡肉组织中利巴韦林总残留量的检测方法。鸡肉经90%乙腈-水溶液提取后,经酸性磷酸酯酶酶解,利用石墨化炭黑(GCB)和十八烷基键合硅胶(C18)净化,采用多反应监测(MRM)-信息依赖扫描(IDA)-增强离子扫描(EPI)模式检测,建立在线EPI谱库检索辅助定性分析、同位素内标法定量分析的方法。目标物质量浓度在1~40 ng/mL范围内呈现良好的线性,线性相关系数大于0. 99,检出限为0. 3μg/kg,在不同浓度水平进行加标实验,回收率在95. 0%~103. 4%之间,相对标准偏差小于3. 4%。方法适用于鸡肉中低浓度利巴韦林残留总量的检测。
A method has been developed for the determination of ribavirin residues in chicken using DSPE-UHPLC-QTRAP-MS/MS.The ribavirin was extracted from chicken samples by 90%acetonitrile-water solution,and then hydrolyzed by acid phosphatase.The extracting solution was purified with GCB and C18,then analysedby MRM(Multi-reaction Monitoring)-IDA(Information Dependent Scanning)-EPI(Enhanced Ion Scanning).Quantitative analysis was established with EPI spectral library retrieval assisted qualitative analysisand isotope internal standard method.The calibration curve of ribavirin was linear in the range of 1-40 ng/mLand the linear correlation coefficient was above 0.99.The detection limit was 0.3μg/kg.The average recoveries ranged from 95.0%to 103.4%,the relative standard deviation was less than 3.4%.The method is suitable forthe determination of low concentration ribavirin residues in chicken.
作者
赵超群
陈万勤
袁堃
陈晶燕
刘柱
ZHAO Chaoqun;CHEN Wanqin;YUAN Kun;CHEN Jingyan;LIU Zhu(Zhejiang Institute for Food and Drug Control,Hangzhou 310052)
出处
《分析试验室》
CAS
CSCD
北大核心
2020年第6期679-683,共5页
Chinese Journal of Analysis Laboratory
基金
浙江省基础公益研究计划分析测试项目(LGC19C200001)资助。
关键词
利巴韦林
净化
分散固相萃取
线性离子阱串联质谱
ribavirin
purification
dispersive solid-phaseextraction
linear ion trap tandem mass spectrometry
作者简介
赵超群,男(1990-),硕士,主管药师。研究方向:食品理化检验,chaoqun199001@126.com;通信作者:刘柱,男(1982-),硕士,高级工程师。研究方向:食品理化检验,zliu82@126.com。
