摘要
目的探讨复发性流产(RSA)孕妇血清25-羟维生素D[25(OH)D]水平与炎症因子、自身免疫抗体及T淋巴细胞亚群分布的相关性分析。方法 2018年1月至2019年12月间在本院进行清宫的复发性流产孕妇40例作为复发性流产组,同期在本院进行人工流产的健康女性40例作为对照组,检测两组孕妇的血清25(OH)D水平,对比不同25(OH)D水平的复发性流产孕妇血清炎症因子白介素-6(IL-6)水平、外周血自身免疫抗体[抗心磷脂抗体(ACA)、抗子宫内膜抗体(AEMAb)、抗HCG抗体(AHCGAb)、抗核抗体(ANA)、甲状腺过氧化物酶抗体(TPOAb)]表达阳性情况及T淋巴细胞亚群[CD3^+、CD3^+CD4^+、CD3^+CD8^+T淋巴细胞]分布情况的差异。结果复发性流产组孕妇血清中25(OH)D的水平低于对照组孕妇,差异有统计学意义(P<0.05)。低25(OH)D组孕妇血清中IL-6水平(11.30±1.67vs 15.62±2.39 pg/mL)低于高25(OH)D组,外周血中ACA、AEMAb、AHCGAb、ANA、TPOAb的阳性率及CD3^+、CD3^+CD4^+T淋巴细胞的分布比例高于高25(OH)D组孕妇。两组间CD3^+CD8^+T淋巴细胞分布比较,差异无统计学意义(P>0.05)。复发性流产孕妇血清25(OH)D水平与炎症因子、自身免疫抗体及T淋巴细胞亚群分布均存在直接相关关系(r=0.384、-0.409、-0.322、-0.295、-0.311、-0.503、-0.437、-0.399)。结论 25(OH)D水平降低在RSA发生过程中扮演重要角色,可能与其影响机体免疫炎症反应的机制相关。
Objective To explore the correlation analysis of serum 25-hydroxyvitamin D[25(OH)D]levels in pregnant women with recurrent miscarriage(RSA)and the distribution of inflammatory factors,autoimmune antibodies and T lymphocyte subsets.Methods From January 2018 to December 2019,40 pregnant women with recurrent miscarriages in our hospital were recruited as the recurrent abortion group,and 40 healthy women who underwent artificial abortion in our hospital during the same period were used as the control group.The difference of serum inflammatory factor interleukin-6(IL-6)level,peripheral blood autoimmune antibody[anticardiolipin antibody(ACA),anti-endometrial antibody(AEMAb),anti HCG antibody(AHCGAb),antinuclear antibody(ANA),thyroid peroxidase antibody(TPOAb)]positive expression and distribution of T lymphocyte subsets[CD3+,CD3+CD4+,CD3+CD8+T lymphocytes]were analyzed and compared.Results Serum level of 25(OH)D in the RSA group was lower than that in the control group(P<0.05).Serum levels of IL-6 of the low 25(OH)D group were lower than those of the high 25(OH)D group.Positive rate of ACA,AEMAb,AHCGAb,ANA,TPOAb and distribution ratio of CD3+、CD3+CD4+T lymphocytes in peripheral blood in the low 25(OH)D group were higher than those in the high 25(OH)D group(P<0.05),and there were no significant difference in distribution ratio of CD3+CD8+T lymphocytes between two groups(P>0.05).Correlation analysis showed that serum 25(OH)D level in RSA women was correlated with the distribution of inflammatory cytokines,autoimmunity antibodies and T lympho-cyte subpopulations.Conclusion The reduction of 25(OH)D level plays an important role in the occurrence of RSA,which may be related to the mechanism that affects the bodys immune and inflammatory response.
作者
郝月兰
刘辉
吕明云
滕怀香
HAO Yuelan;LIU Hui;LV Mingyun;TENG Huaixiang(Department of Reproductive Medicine,Rizhao City Maternal and Child Health Hospital Rizhao,Shandong,China,276826;Department of Reproductive Medicine,Rizhao People's Hospital,Rizhao,Shandong,China,276826)
出处
《分子诊断与治疗杂志》
2020年第6期799-802,共4页
Journal of Molecular Diagnostics and Therapy
关键词
复发性流产
25-羟维生素D
炎症因子
自身免疫抗体
T淋巴细胞亚群
Recurrent spontaneous abortion
25-hydroxyvitamin D
Inflammatory cytokines
Autoimmune antibody
T lymphocyte subsets
作者简介
通信作者:郝月兰,E-mail:hyl6506@126.com。
