摘要
目的探索不同剂量顺铂(CP)腹腔注射建立化疗损伤性卵巢早衰(POF)大鼠模型的方法。方法取48只雌性未生育Wistar大鼠分为6组,每组8只。在不同时间点腹腔注射不同剂量CP。对照组(A组)无处理;CP腹腔小剂量(1.5 mg/kg)注射1组(B组)、小剂量(2.0 mg/kg)注射2组(C组)和小剂量(2.5 mg/kg)注射3组(D组),均每日1次,连续5 d;CP腹腔注射大剂量1组(E组),4 mg/kg,间隔一周再次注射4 mg/kg;CP腹腔注射大剂量2组(F组),4 mg/kg,间隔一周注射6 mg/kg,建立大鼠化疗损伤性POF模型。观察大鼠一般情况、动情周期,检测体质量变化和血清抗苗勒氏管激素(AMH)水平,以及卵巢病理学改变和各级卵泡数量。结果与A组比较,各给药组大鼠动情周期明显紊乱,体质量明显下降,血清AMH水平也有明显降低(P<0.05),其中E组下降最为明显;各模型组大鼠卵巢各级卵泡数均有显著减少(P<0.05),其中E组、F组各级卵泡数量减少更为明显(P<0.01)。结论大鼠腹腔注射4 mg/kg CP,间隔一周再次注射,诱导化疗损伤性POF的造模效果更好,造模方法简便。
Objective To investigate a method for establishing a rat model of chemotherapyinduced premature ovarian failure(POF)in rats by intraperitoneal injection with different dosages of cisplatin.Method Fourty-eight unfertiled female Wistar rats were randomly divided into 6 groups with 8 rats in each group.Different doses of cisplatin was injected intraperitoneally at different time points incluing:(1)control group(group A)without treatment,(2)cisplatin intraperitoneal small dose injection group 1(group B)(1.5 mg/kg once daily for 5 days),(3)cisplatin intraperitoneal small dose injection group 2(group C)(2.0 mg/kg once daily for 5 days),(4)cisplatin intraperitoneal small dose injection group 3(group D)(2.5 mg/kg once daily for 5 days),(5)cisplatin intraperitoneal high dose injection group 1(group E)(4 mg/kg once in a week,after a week,re-injection,on total for twice)and(6)cisplatin intraperitoneal high dose injection group 2(group F)(4 mg/kg once in a week after a week,re-injection for 6 mg/kg,on total for twice),to establish a rat model of chemotherapyinduced injury POF.The general condition,estrous cycle,body weight changes,serum anti-Müllerian hormone(AMH)levels,ovarian pathological changes,and number of follicles at various levels were observed.Result Compared with the control group(group A):the estrous cycle of the rats in each group was significantly disordered,the body weight and serum AMH levels were significantly decreased(P<0.05),in which,the group E was decreased most obviously.The number of follicles in the ovary of each group was also significantly decreased(P<0.05),but the number of follicles in the groups E and F was more significantly decreased.Conclusion Rats can be injected intraperitoneally with 4 mg/kg cisplatin once a week,at intervals of one week,for two weeks.This mode method of chemotherapy-induced POF is more effective,and the method of modeling is simple.
作者
董若曦
朱小丹
樊伯珍
许文娟
Dong Ruoxi;Zhu Xiaodan;Fan Bozhen;Xu Wenjuan(Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China;Department of Gynecology,Putuo Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200062,China;Putuo Central Medical College,Anhui Medical University,Shanghai 200062,China)
出处
《实验动物与比较医学》
CAS
2020年第2期104-109,共6页
Laboratory Animal and Comparative Medicine
基金
上海市科委基金资助项目(18411970500)
上海市加强公共卫生体系建设三年行动计划(GWIV-26)
上海中医药大学附属普陀医院引进人才基金(2016317A)
上海中医药大学附属普陀医院重点专科建设项目(2016108A)。
关键词
顺铂
腹腔化疗
卵巢早衰
抗苗勒氏管激素
动物模型
Cisplatin
Intraperitoneal chemotherapy
Premature ovarian failure
Anti-Mülerian hormone
Animal model
作者简介
董若曦(1994-),女,博士研究生。E-mail:2403425354@qq.com;通信作者:许文娟(1967-),女,硕士,副主任医师,研究方向:中西医结合妇产科学。E-mail:farewelltoyou@126.com。
