摘要
目的:研究运动干预对高脂饲养的小鼠炎性细胞因子和脂肪因子的影响。方法:将SPF级C57BL/6小鼠41只(雄性20只,雌性21只)随机分成正常对照组(NC组,n=11,雄性5只,雌性6只)和高脂饮食组(HFD组, n=30,雄性15只,雌性15只),分别以普通饲料和高脂饲料喂养。9周后,将HFD小鼠25只随机分为肥胖运动组(OE组,n=13,雄性5只,雌性8只)与肥胖对照组(OC组,n=12,雄性5只,雌性7只)。OE组进行为期8周无负重游泳训练。8周后每组随机挑选8只(雄性4只,雌性4只)小鼠,用ELISA法测定其血清脂联素(APN)、网膜素、瘦素、内脂素、C-反应蛋白(CRP)、白细胞介素-6(IL-6)、肿瘤坏死因子α(TNF-α)水平。结果:①OE组与OC组小鼠体重均显著高于NC组(P<0.01),OE组体重显著低于OC组(P<0.05)。②与NC组比较,OC组小鼠血清瘦素、内脂素、CRP、IL-6、TNF-α水平均显著上升(P<0.01);脂联素和网膜素有下降趋势,但差异不显著(P>0.05)。与OC组相比,OE组小鼠血清脂联素(P<0.05)、网膜素(P<0.01)水平显著上升;内脂素水平下降(P<0.05);瘦素、CRP、IL-6、TNF-α水平均显著下降(P<0.01)。③小鼠血清脂联素与血清IL-6有微弱的负相关关系(P<0.05);血清脂联素与网膜素有较弱的正相关关系(P<0.05);小鼠血清网膜素与血清CRP有中等程度的负相关关系(P<0.01);小鼠血清瘦素、内脂素均与血清IL-6、TNF-α有中等程度的正相关关系(P<0.01)。结论:8周游泳运动干预可以通过刺激能抑制炎症因子的脂联素、网膜素的分泌及抑制促炎因子瘦素、内脂素的分泌有效减轻肥胖小鼠炎症反应。
Objective:To investigate the effect of exercise intervention on inflammatory cytokines and adipokines in mice with high-fat diet.Method:Forty-one SPF C57BL/6 mice(20 males and 21 females)were randomly divided into normal control group(NC,n=11,5 males,6 females)and high-fat diet group(HFD,n=30,15 males and 15 females),and fed with ordinary food and high fat diet separately.After 9 weeks,twenty-five obese mice were randomly divided into obesity exercise intervention group(OE,n=13,5 males,8 females)and obesity control group(OC,n=12,male 5,7 females),and continued the high fat diet.The OE group was trained for 8 weeks with weightbearing swimming.After the 17 weeks program,the APN,omentin,leptin,visfatin,CRP,IL-6,TNF-αin serum were measured by ELISA for all rats.Result:①Compared with NC,mice in both OC and OE’s body weight increased(P<0.01).Compared with OC,mice in OE’s weight increased more(P<0.05).②Compared with NC,the serum leptin,visfatin,CRP,IL-6 and TNF-αlevels in OC increased significantly(P<0.01),the serum APN and omentin decreased,but not significant(P>0.05).Compared with the OC,the serum APN levels(P<0.05)and the serum omentin levels(P<0.01)of the OE increased,the levels of visfatin decreased(P<0.05),the levels of leptin,CRP,IL-6,TNF-αdecreased significantly(P<0.01).③There was a weak negative correlation between serum APN levels and serum IL-6 in mice(P<0.05).Serum APN levels are weak positively correlated with serum omentin in mice(P<0.05).Serum omentin levels are intermediate negatively correlated with serum CRP(P<0.01),serum leptin and serum visfatin were intermediate positively correlated with both serum IL-6 and TNF-α(P<0.01).Conclusion:The 8-weeks swimming exercise intervention can effectively reduce the inflammatory response of obese mice and slow down the inflammatory process by stimulating the secretion of the factors APN and omentin which inhibit inflammatory factors and inhibiting the proinflammatory cytokines Leptin and visfatin.
作者
张旖
张洪兵
ZHANG Yi;ZHANG Hongbing(Nanjing University of Chinese Medicine,210023)
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2020年第3期301-305,共5页
Chinese Journal of Rehabilitation Medicine
关键词
肥胖
运动干预
炎性细胞因子
脂肪因子
obseity
exercise intervention
inflammatory cytokines
adipokines
作者简介
第一作者:张旖,女,硕士研究生。
