摘要
目的观察抵抗素基因(Retn基因)在肥胖小鼠脂肪组织中的表达,及其蛋白(resistin蛋白)对小鼠肌肉组织葡萄糖摄取率的影响,探讨肥胖、抵抗素与胰岛素抵抗可能的相关性。方法用高脂肪和高能量型的小鼠饲料喂养C57BL/6J小鼠,诱导其产生肥胖伴胰岛素抵抗模型,22周后测定小鼠的Lee’s指数(即体质指数,BMI)以及血糖和血浆胰岛素的浓度,然后进行葡萄糖耐量试验,并与正常小鼠作对照,以评价肥胖小鼠是否出现了胰岛素抵抗和葡萄糖耐量损伤。实时荧光定量RT-PCR比较肥胖组小鼠(n=10)与正常组小鼠(n=5)白色脂肪细胞Retn基因的表达。最后将抵抗素蛋白与小鼠骨骼肌细胞共培养,测定其对肌肉组织葡萄糖摄取率的影响。结果采用高脂饲料成功地诱导出了肥胖伴胰岛素抵抗的小鼠模型;肥胖小鼠的白色脂肪组织内Retn基因的表达显著高于正常小鼠(P<0.01);将抵抗素蛋白与小鼠肌肉组织在体外共培养,不论是否有胰岛素刺激,均能显著抑制小鼠肌肉组织对葡萄糖的摄取率(P<0.05)。结论肥胖小鼠体内Retn基因的高表达可能是导致骨骼肌对葡萄糖的摄取率降低,进而诱发胰岛素抵抗的因素之一。
Objective：To investigate the expression of resistin （Retn） gene in the adipose tissues of obese mouse and its influence on the skeletal muscle glucose uptake, in an effort to understand the correlation between obese, resistin, and insulin resistance. Methods： The obese and insulin resistance model was induced with high-fat diet in C57BL/6J mice. Twenty-two weeks later the Lee＇s index （BMI） and the concentrations of blood glucose and plasma insulin were determined. Glucose tolerance test was carried out to verify the appearance of insulin resistance and the impair to glucose tolerance, with normal mice taken as control. Retn mRNA expression in the adipose tissues of model mice （n= 10） and control mice （n= 5） was detected by Real-time RT-PCR. The resultant resistin protein was co-cultured with mouse skeletal muscle to assess its influence on glucose uptake. Results： An obese and insulin resistance mouse model was successfully induced with high fat diet. The Retn gene expression in adipose tissues was significantly higher in the obese mice than that in normal control mice（P〈0.01）. The resultant resistin protein had a significant inhibitory effect on the glucose uptake by skeletal muscle with or without insulin（P〈0.05）. Conclusion： It is suggested that the overexpression of Retn gene might be one of the reasons responsible for the decrease of glucose uptake by skeletal muscle and the subsequent insulin resistance in the diet-induced obese mice.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2006年第12期1351-1354,共4页
Academic Journal of Second Military Medical University
基金
国家自然科学基金(30370802)~~
作者简介
李雅慧，博士，副主任医师．Corresponding author．E-mail：tlyahui661021@yahoo．com．cn
