摘要
目的:观察连翘酯苷A(FSA)对哮喘小鼠气道炎症的影响,并探究其可能的作用机制。方法:40只雄性BALB/c小鼠随机分为5组:CON组、OVA组、FSA 15组、FSA 30组和DEX组,每组8只。卵清蛋白(OVA)诱导建立哮喘小鼠模型,并给予相应剂量的FSA处理。最后一次OVA激发24 h后,处死小鼠,取各小鼠支气管肺泡灌洗液(BALF)及双肺。Diff-Quik染色检测小鼠BALF中各类炎症细胞数量,ELISA和Western blot检测FSA对小鼠BALF及肺组织中IL-4、IL-5、IL-13和IFN-γ水平的影响。HE、PAS和Masson染色观察小鼠肺组织的病理学变化。免疫组化法和Western blot检测肺组织中p-p38 MAPK及NF-κB p65的表达情况。结果:FSA处理可显著改善哮喘小鼠肺组织中炎症细胞的浸润、气道上皮杯状细胞增生、气道黏液过量分泌及胶原沉积,FSA处理也可减少哮喘小鼠BALF中各炎症细胞及总炎症细胞数量,降低哮喘小鼠BALF及肺组织中IL-4、IL-5、IL-13水平并增加IFN-γ水平;同时FSA可抑制p38 MAPK的磷酸化进而抑制NF-κB活化。结论:FSA可抑制哮喘小鼠的气道炎症反应,其机制可能与抑制p38 MAPK/NF-κB信号通路有关。
Objective:To investigate the effects of forsythiaside A(FSA)on airway inflammation in asthmatic mice,and to explore its possible mechanism of action.Methods:40 female BALB/c mice were randomly divided into five groups with 8 mice in each group:CON group,OVA group,FSA 15 group,FSA 30 group,and DEX group.An ovalbumin(OVA)-induced asthma mouse model was established and administered the corresponding dose of drug treatment in each group of mice.After the last OVA challenge for 24 h,the mice were sacrificed and bronchoalveolar lavage fluid(BALF) and both lungs were taken from each mouse.Diff-Quik staining was used to detect the number of various inflammatory cells and total inflammatory cells in bronchoalveolar lavage fluid(BALF).The effects of FSA on the levels of interleukin-4,5,13(IL-4,5,13) and interferon-γ(IFN-γ) in BALF and lung tissue of mice were detected by ELISA and Western blot.The pathological changes of lung tissue were observed by HE,PAS and Masson staining.The contents of p-p38 MAPK and NF-κB p65 in lung tissue were detected by immunohistochemistry and Western blot.Results:Staining results show that FSA could significantly reduce the infiltration of inflammatory cells in lung tissue of asthmatic mice and airway mucus secretion and collagen deposition in lung tissue of asthmatic mice;FSA could significantly reduce the number of various inflammatory cells and total inflammatory cells in BALF,and decreased the content of IL-4,IL-5 and IL-13,and increase the content of IFN-γ in BALF and lung tissue of OVA-induced asthmatic mice.At the same time,FSA could inhibit phosphorylation of p38 MAPK and nuclear translocation of NF-κB.Conclusion:FSA could inhibit airway inflammation in asthmatic mice,and its mechanism may be related to inhibition of p38 MAPK/NF-κB signaling pathway.
作者
林星
李俊峰
车楠
李良昌
李莉
LIN Xing;LI Jun-Feng;CHE Nan;LI Liang-Chang;LI Li(Department of Thoracic Surgery,Affiliated Hospital of Yanbian University,Yanji 133000,China)
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2019年第24期2971-2974,2979,共5页
Chinese Journal of Immunology
基金
国家自然科学基金项目(81560004)
吉林省科技厅项目(20180101141JC)资助
作者简介
林星,男,硕士,主治医师,主要从事肺部发病疾病机制研究,E-mail:18426678@qq.com;通讯作者/指导教师:李莉,女,硕士,讲师,主要从事过敏性疾病研究,E-mail:lili@ybu.edu.cn。
