摘要
目的探讨温阳散瘀平喘方对哮喘模型小鼠核因子κ-B(NF-κB)p65、信号转导和转录活化因子(STAT6)及相关炎症因子水平的影响。方法将40只BALB/c雄性小鼠随机分为空白对照组、哮喘模型组、地塞米松组、温阳散瘀平喘方组,每组10只。除空白对照组小鼠外,其余各组建立哮喘模型,在此基础上给予相应药物干预。28 d后取材,HE染色法观察肺组织病理学改变,ELISA法测定IL-4、IL-13、IFN-γ与IgE水平,Western blot法检测p65、STAT6蛋白含量。结果与空白对照组比较,哮喘模型组IL-4、IL-13水平与p65、STAT6含量明显升高(P<0.01),IFN-γ水平显著降低(P<0.01);与哮喘模型组比较,地塞米松组及温阳散瘀平喘方组IL-4、IL-13水平,地塞米松组p65、STAT6,温阳散瘀平喘方组STAT6含量显著降低(P<0.01),地塞米松组及温阳散瘀平喘方组IFN-γ水平显著升高(P<0.01),温阳散瘀平喘方组p65含量降低(P<0.05);与地塞米松组比较,温阳散瘀平喘方组IL-4水平升高(P<0.05),IL-13水平显著升高(P<0.01),IFN-γ水平显著降低(P<0.01),而地塞米松组及温阳散瘀平喘方组p65、STAT6含量差异无统计学意义(P>0.05)。结论温阳散瘀平喘方可以降低肺组织内p65、STAT6蛋白含量,进而影响肺内炎症因子IL-4、IL-13、IFN-γ分泌;温阳散瘀平喘方可能是通过调节NF-κB、Janus激酶/信号转导与转录激活子(JAK/STAT)信号通路,缓解哮喘模型小鼠的气道炎症。
Objective To investigate the effect of Wenyang Sanyu Pingchuan recipe on the levels of nuclear factor-κB(NF-κB) p65, signal transducers and activators of transcription 6(STAT6) and related inflammatory factors in asthmatic mice. Methods Forty male BALB/c mice were randomly divided into blank control group, asthma model group, dexamethasone group and Wenyang Sanyu Pingchuan recipe group, with 10 mice in each group. Except the blank control group, mice in other groups established the asthma model, and were given corresponding drug intervention. HE staining was used to observe the histopathological changes of the lung, the levels of Interleukin 4(IL-4), Interleukin 13(IL-13), interferon-γ(IFN-γ) and Immunoglobulin E(IgE) were determined by ELISA, and the protein levels of p65 and STAT6 were detected by Western blot after 28 days. Results Compared with the blank control group, the levels of IL-4, IL-13 and p65, STAT6 in the model group increased significantly(P<0.01), and the level of IFN-γ significantly decreased(P<0.01). Compared with model group, the levels of IL-4 and IL-13 in dexamethasone group and Wenyang Sanyu Pingchuan recipe group, the levels of p65 and STAT6 in dexamethasone group and the level of STAT6 in Wenyang Sanyu Pingchuan recipe group significantly reduced( P<0.01). Compared with model group,the levels of IFN-γ in two drug delivery groups significantly elevated( P<0.01),and the level of p65 in Wenyang Sanyu Pingchuan recipe group reduced( P<0.05). Compared with the dexamethasone group, the level of IL-4 in Wenyang Sanyu Pingchuan recipe group increased( P<0.05),the level of IL-13 significantly increased( P<0.01), and the level of IFN-γsignificantly decreased( P<0.01),but there was no significant difference in the levels of p65 and STAT6 between dexamethasone group and Wenyang Sanyu Pingchuan recipe group( P > 0. 05). Conclusion Wenyang Sanyu Pingchuan Recipe could reduce the levels of p65 and STAT6 in lung tissue,thus affecting the secretion of inflammatory factors IL-4,IL-13 and IFN-γ in lung. Wenyang Sanyu Pingchuan recipe may relieve airway inflammation in asthma model mice by regulating NF-κB and Janus kinase/signal transduction and transcription activator( JAK/STAT) signaling pathways.
作者
蒋沈华
白莉
李利清
朴香
武明云
虞坚尔
JIANG Shenhua;BAI Li;LI Liqing;PIAO Xiang;WU Mingyun;YU Jian’er(Department of Pediatrics,Shanghai Municipal Hospital of Traditional Chinese Medicine Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200071,China;Pediatric Institute of Shanghai Traditional Chinese Medicine Academy,Shanghai 200071,China)
出处
《上海中医药杂志》
2020年第5期93-97,共5页
Shanghai Journal of Traditional Chinese Medicine
基金
国家自然科学基金青年项目(81603662)
上海市科委青年科技英才扬帆计划资助项目(16YF1410800)
上海市卫健委虞坚尔名老中医学术经验研究工作室项目(SHGZS-2017001)
作者简介
蒋沈华,女,博士研究生,主要从事中医儿科临床研究工作。;通信作者:虞坚尔,主任医师,教授,博士研究生导师。E-mail:jianeryu@hotmial.com
