摘要
筛选提取荞麦皮多糖的温度和乙醇体积分数,对多糖进行红外光谱分析、光学显微镜观察其形貌、紫外光谱分析、HPLC分析其单糖组成,并测定多糖对铁氰化钾的还原能力、羟基自由基和1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除能力。结果表明:80℃水提取的粗多糖质量百分数显著高于其他温度(P<0.05),且温度对多糖官能团无明显改变;乙醇体积分数为95%时,粗多糖沉淀量显著高于其他各组(P<0.05);多糖分子是絮状、链状结构,在192nm处有强烈紫外吸收;荞麦皮多糖主要由甘露糖、核糖、葡萄糖醛酸、葡萄糖、半乳糖、阿拉伯糖组成,其相对质量百分数分别为6.47%、1.18%、9.66%、52.63%、17.26%、12.8%;多糖对铁氰化钾还原能力、羟基自由基和DPPH自由基清除能力具有显著的浓度依赖性(P<0.05)。荞麦皮多糖主要由6种单糖组成,其抗氧化效果良好,可用作天然的抗氧剂。
The temperature and ethanol concentration to preferably extract buckwheat husk polysaccharides were screened,followed by observing the morphology and composition of the polysaccharides.Besides,the antioxidative capacity of the polysaccharides was determined by potassium ferricyanide assay,hydroxyl radical assay,and DPPHo assay.The results showed that the yield of crude polysaccharides was significantly higher at 80℃and 90℃(P<0.05),and temperature had no significant effects on functional groups of polysaccharides.Moreover,the precipitate amounts of crude polysaccharides were significantly higher with 95%ethanol(P<0.05).The polysaccharides had floc and chain shapes,and they had strong ultraviolet absorption at 192 nm.The primary monosaccharide compositions of the polysaccharides were mannose,ribose,glucuronic acid,glucose,galactose,and arabinose,with a mass fraction of 6.47%,1.18%,9.66%,52.63%,17.26%,and 12.8%,respectively.Furthermore,the polysaccharides had significant concentration-dependent antioxidative effects(P<0.05).In conclusion,buckwheat husk polysaccharides compose of six monosaccharides,and they have good antioxidant activity and can therefore be used as a natural antioxidant.
作者
刘瑶
王新然
赵悦
李林强
LIU Yao;WANG Xinran;ZHAO Yue;LI Linqiang(College of Food Engineering and Nutritional Science,Shaanxi Normal University,Xi′an 710119,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2019年第13期134-140,共7页
Food and Fermentation Industries
基金
陕西省重点研发计划项目(2016NY-212,2017NY-060,2018ZDXM-NY-094)
关键词
荞麦皮
多糖
单糖组成
抗氧化
buckwheat husk
polysaccharides
monosaccharide compositions
antioxidant
作者简介
第一作者:刘瑶,硕士研究生;通讯作者:李林强,博士,E-mail:lilinq@snnu.edu.cn。
