摘要
研究用碱溶液(0.3mol/L NaOH)提取荞麦水溶性多糖,分别用料液比(1∶30,1∶40,1∶50)和提取时间(1h,2h,3h),提取温度(70℃,80℃,90℃)3因素3水平正交法提取多糖,并研究了这3个因素对多糖得率的影响,方差分析表明温度对多糖提取影响较显著(P<0.1),料液比和时间影响不显著。在本实验条件下,荞麦多糖的得率达25.16%。同时用Sevag方法除蛋白,石油醚回流脱脂,淀粉酶,纤维素酶除去淀粉和纤维素,双氧水脱去色素对多糖进行了纯化。
This study was on extracting polysaecharide from buckwheat by orthogonal design. Selected three factors （Solid- solvent ratio： 1 ： 30,1 ： 40,1 ： 50; extracting temperature： 70℃,80℃ ,90℃ and extracting time： lh,2h, 3h）. The results showed extracting temperature was significant （P〈0. 1） to extract buckwheat polysaccharide , then Solid-solvent ratio and extracting time were not significant（P〉0. 1）. under this condition, polysaccharide extracting rate was 25.16%. Meanwhile, removed protein using Sevag method, removed oil using petroleum ether , removed amylum and cellulose using amylase and cellulase, removed pigment using H2O2 to purify polysaccharide.
出处
《中国林副特产》
2008年第5期32-33,共2页
Forest By-product and Speciality in China
基金
安徽工程科技学院青年基金资助(2003Q004)
关键词
荞麦
碱性溶液
多糖
正交
Buckwheat
alkali method
polysaccharide
orthogonal
