摘要
目的:研究野山杏果肉总有机酸(total organic acids from wild apricot,TOAWA)对高脂血症大鼠的降血脂作用及机理。方法:建立高脂血症大鼠模型,以灌胃血脂康大鼠为阳性对照组,利用试剂盒测定血脂浓度和载脂蛋白质量浓度;利用实时荧光定量聚合酶链式反应(real-time polymerase chain reaction,qPCR)测定大鼠肝脏过氧化物酶体增殖物激活受体α(peroxisome proliferator-activated receptor-α,PPAR-α)和低密度脂蛋白受体(low-density lipoprotein receptor,LDL-R)的基因表达水平;制作实验大鼠的肝脏组织切片,观察其对肝脏组织结构的影响;探究TOAWA对高脂血症大鼠血脂的影响及作用机理。结果:TOAWA能极显著降低灌胃高脂乳剂实验大鼠的总胆固醇、甘油三酯和低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)浓度(P<0.01),显著降低载脂蛋白(apoprotein,Apo)B质量浓度(P<0.05),极显著升高ApoA-I质量浓度和ApoA-I/Apo-B(P<0.01),显著升高高密度脂蛋白胆固醇浓度(P<0.05)。qPCR结果显示,灌胃高脂乳剂实验大鼠肝脏PPAR-α和LDL-R基因表达量降低,给予TOAWA干预后,实验大鼠肝脏PPAR-α和LDL-R基因表达量有明显的升高。肝脏组织切片的观察结果显示,TOAWA可明显改善肝功能。结论:TOAWA能调节高脂血症大鼠的血脂浓度,其作用与TOAWA调节Apo质量浓度、上调肝脏PPAR-α和LDL-R基因表达量及改善肝功能有关。
Objective:To investigate the effect of total organic acid from wild apricot(TOAWA)on blood lipids and to explore the hypolipidemic mechanism of TOAWA in hyperlipidemic rats.Methods:A rat model of hyperlipidemia was established by orally feeding high-fat emulsion.Rats administered with Xuezhikang,a hypolipidemic agent were used as a positive control group.Commercial kits were employed to measure blood lipids and apolipoprotein.The mRNA expression levels of peroxisome proliferator-activated receptor-α(PPAR-α)and low-density lipoprotein receptor(LDL-R)in liver were measured by real-time polymerase chain reaction(qPCR),and the effect of TOAWA on blood lipids and pathological changes in liver was evaluated.Furthermore,the hypolipidemic mechanism of TOAWA was explored.Results:TOAWA could significantly decrease the levels of total cholesterol,triglycerides,low-density lipoprotein cholesterol(LDL-C)(P<0.01)and apoprotein B(Apo-B)(P<0.05),and increase the levels of high-density lipoprotein cholesterol(HDL-C)(P<0.05),apoprotein A-1(ApoA-I)and ApoA-I/Apo-B ratio in the serum of hyperlipoidemic rats(P<0.01).The mRNA expression levels of PPAR-αand LDL-R were decreased in the liver of hyperlipidemic rats compared with normal ones,and they were increased by intervention with TOAWA.Besides,the degree of hepatic steatosis was alleviated by TOAWA.Conclusion:TOAWA can decrease blood lipid levels in hyperlipoidemic rats by altering the level of apolipoprotein in serum and upregulating the mRNA expression levels of PPAR-αand LDL-R in liver and improving liver function.
作者
阿依姑丽.艾合麦提
王妙颖
彭禛菲
冯雅丽
王小燕
古扎丽努尔.艾尼
Aygul·AHMAT;WANG Miaoying;PENG Zhenfei;FENG Yali;WANG Xiaoyan;Guzalnur·GENI(College of Food Science and Pharmacy,Xinjiang Agricultural University,ürümqi 830052,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2018年第21期171-176,共6页
Food Science
基金
国家自然科学基金地区科学基金项目(31460393)
关键词
野山杏果肉总有机酸
高脂血症
血脂
作用机制
total organic acids from wild apricot
hyperlipidemia
blood lipids
hypolipidemic mechanism
作者简介
阿依姑丽·艾合麦提(1965—),女,副教授,硕士,研究方向为天然产物功能性成分。E-mail:glcpau@163.com。
