摘要
目的:研究中国野生菰米对高脂膳食诱导的胰岛素抵抗大鼠血脂浓度以及炎性因子水平的影响。方法:50只雄性SD大鼠随机分成阴性对照组,胰岛素抵抗模型组,高、低剂量菰米组和米面组。连续喂养8周,实验前、后测定各组大鼠的体质量及总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)和高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C)浓度,同时测定高敏C反应蛋白(hypersensitiveC-reactiveprotein,hs-CRP)、肿瘤坏死因子-α(tumor necrosisfactor,TNF-α)、白介素-6(interleukin-6,IL-6)含量,用SPSS18.0软件对数据进行统计分析。结果:高剂量菰米组大鼠与米面组及胰岛素抵抗模型组大鼠相比,HDL-C浓度显著上升(P<0.05),体质量和TC、TG、LDL-C浓度均显著下降(P<0.05)。与胰岛素抵抗模型组相比,高剂量菰米组的TNF-α及IL-6含量显著下降(P<0.05)。与阴性对照组相比,低、高剂量菰米组IL-6含量均显著升高(P<0.05)。结论:中国野生菰米具有改善高脂膳食诱导的大鼠胰岛素抵抗作用,该作用的机制可能是通过降低血脂浓度,调节炎性因子的表达,从而改善胰岛素抵抗。
Objective:To evaluate the effect of dietary supplementation of wild rice seeds(Zizania latifolia)on blood lipids and inflammatory factor levels in some tissues of high-fat fed rats with insulin resistance.Methods:Totally 50 SD rats were randomly divided into 5 groups:negative control,insulin resistance model,high-dose wild rice,low-dose wild rice and rice flour groups.Before and after these animals were fed for 8 weeks,body mass and total cholesterol(TC),triglyceride(TG),high-density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL-C),hypersensitive C-reactive protein(hs-CRP),tumor necrosis factor(TNF-α),and interleukin-6(IL-6)levels were measured.The SPSS 18.0 software was used to analyze the experimental data.Results:Compared with the insulin resistance model and rice flour groups,body mass and the levels of TC,TG and LDL-C in rats administered with wild rice at both doses declined significantly(P<0.05),and the level of HDL-C increased significantly(P<0.05).Compared with the insulin resistance model group,the level of TNF-αand IL-6 in the high-dose wild rice group significantly decreased(P<0.05).Both the low-dose and high-dose groups showed a significantly increased level of IL-6 in comparison with the negative control group(P<0.05).Conclusion:Wild rice seeds have the potential to improve insulin resistance in high-fat diet fed rats,likely by reducing blood lipids and regulating the expression of inflammatory cytokines.
作者
王菁
刘洋
张红
王少康
孙桂菊
WANG Jing;LIU Yang;ZHANG Hong;WANG Shaokang;SUN Guiju(Department of Nutrition and Food Hygiene,School of Public Health,Southeast University,Nanjing 210009,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2018年第21期166-170,共5页
Food Science
基金
国家自然科学基金面上项目(81673162)
国家自然科学基金青年科学基金项目(81202195)
关键词
野生菰米
胰岛素抵抗
血脂
炎性因子
wild rice seeds
insulin resistance
blood lipids
inflammatory cytokines
作者简介
王菁(1994—),女,硕士研究生,研究方向为全谷物与营养。E-mail:220163003@seu.edu.cn;通信作者:王少康(1975—),男,副教授,博士,研究方向为营养与肿瘤。E-mail:shaokangwang@seu.edu.cn。
