摘要
本实验通过静态吸附量和吸附率的测定确定血管紧张素转移酶(ACE)抑制肽分离富集的最适操作条件,考察了pH值、盐浓度和乙醇浓度对ACE抑制肽吸附性能的影响。结果表明,上样pH2.0对ACE抑制肽的脱盐效果最佳,70%乙醇作为洗脱剂时效果最好,并且原样液的盐浓度保持不变。因此DA201-C大孔吸附树脂综合性能较好,适合于ACE抑制肽的分离纯化。
In this study, static adsorption capacity and desorption rate were measured to confirm the optimum manipulationparameters of macroporous resin for separating angiotensin converting enzyme inhibitory peptide (ACE-inhibitory peptide). Theresults showed that the best pH of sample for the desalination of ACE-inhibitory peptide is 2.0, and when 70% ethanol is usedas eluent, the elution effect is the best and the salt concentration of sample is kept constant. In conclusion, DA201-C macroporousresin has excellent ...
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第5期20-23,共4页
Food Science
基金
国家高技术研究发展计划项目(2007AA10Z320)
江苏省自然科学基金项目(BK2005137)
江苏省高校高新技术产业发展项目(JHB06-11)
关键词
大孔吸附树脂
ACE抑制肽
静态吸附
macroporous resin
ACE-inhibitory peptide
static adsorption
