摘要
人参皂苷Re是人参的主要药理活性成分,具有抑制癌细胞增长、阻止癌细胞转移及保护神经等重要生理作用,稀有人参皂苷Rh1在抗癌方面的疗效更为显著。为获得较高含量的Rh1,微生物转化是目前较为有效途径。该研究从人参种植土中筛选可转化常量人参皂苷Re为稀有人参皂苷Rh1的目的菌株S329,以西洋参提取物为反应底物进行微生物发酵实验,利用高效液相色谱(HPLC)法对人参皂苷Re及其发酵产物进行分析。结果表明,通过对菌株形态学观察和18S r DNA测序分析,且通过在NCBI数据库上的Blast比对分析,鉴定高效菌株S329属于溜曲霉(Aspergillus tamarii),人参皂苷Re转化人参皂苷Rh1的转化率为27.65%。
Ginsenoside Re is the major pharmacological active components of ginseng, and it has the important physiological functions including inhibiting the growth and metastasis of cancer cells, protecting nerves and so on. The curative effect of rare ginsenoside Rh1 has more significant effect on anti-cancer. At present, microbial transformation is regarded as an effective approach to obtain the higher concentration ginsenoside Rh1. In this experiment, the target strain S329 was screened from the ginseng planting soil for the use of constant ginsenoside Re to rare ginsenoside Rh1 by microbial transformation. American ginseng extract was served as the fermentation substrate for the microbial fermentation, and ginsenoside Re and the fermentation products were analyzed by HPLC. By strains morphological observation and 18 S r DNA sequencing analysis, Blast comparison analysis on NCBI database, the strain was identified as Aspergillus tamarii, and the conversion rate of ginsenoside Re to Rh1 was 27.65%.
出处
《中国酿造》
CAS
北大核心
2017年第11期114-117,共4页
China Brewing
基金
辽宁省教育厅项目(L2014106)
作者简介
张丽娜（1991-）,女,硕士研究生,研究方向为天然药物的分离.;通讯作者：吴秀红（1968-）,女,教授,博士,研究方向为天然药物制备与分离.
