摘要
对橡子粉酶法制备低聚异麦芽糖的工艺过程进行优化,以期提高低聚异麦芽糖中异麦芽糖、潘糖和异麦芽三糖的含量。采用Box-Benhnken响应面法,优化以耐高温α-淀粉酶液化橡子粉的工艺条件。最佳的液化工艺条件为:以DE值13%为最佳液化指标,液化时间31 min、液化温度95℃、液化p H6.6、酶添加量13 U/g,结合生产实际最佳条件下的DE值为12.91%;继而采用正交实验,优化以普鲁兰酶、β-淀粉酶糖化橡子粉液化液的工艺条件,得到最佳的糖化工艺条件为:普鲁兰酶添加量25 U/g、β-淀粉酶添加量130 U/g、温度60℃、时间10 h,在此最佳工艺条件下糖化转苷后的(IG2+P+IG3)含量为36.11%±0.17%;转苷工艺过程的α-葡萄糖转苷酶最佳添加量为1.5 U/g,最佳条件下异麦芽糖、潘糖、异麦芽三糖含量之和为36.27%±0.18%。
With the acorn starch as raw material, the process of preparation of isomalto-oligosaecharide by enzymatic methods were optimized in order to increase the content of isomaltose, panose and isomaltotriose of isomalto-oligosaccharide. Box- Benhnken response surface analysis method was used to optimize the process conditions of liquefying acorn starch with thermostable a-amylase. The results showed that the DE value of 13% as the best liquefaction index, the optimal liquefaction conditions were 31 rain of liquefaction time ,95 ℃ of liquefaction temperature,6.6 of liquefying pH value, 13 U/g of thermostable a-amylase,at this point, in combination with the production practice, the value of the liquefied liquid DE was 12.91%, then through single factor experiments, the process conditions of saccharifying acorn starch liquefying liquor with pullulanase and glucoamylase were investigated, the saccharification conditions were optimized to be 25 U/g of pullulanase, 130 U/g of glucoamylase ,60 ℃ of saccharification temperature and 10 h of saccharification time, at this optimal condition, the content of（ IG2 ＋ P ＋ IG3 ）was predicted to 36.11% ＋ 0.17%, the optimum concentration of α-glucosidase in transglycosylation process was 1.5 U/g, under the optimal condition, the total content of isomaltose, panose, and isomahotriose were 36.27 % ± 0.18 %.
作者
张智
李晴
化洪苓
尹文哲
李杰
马建章
ZHANG Zhi LI Qing HUA Hong-ling YIN Wen-zhe LI Jie MA Jian-zhang(Northeast Forestry University,Harbin 150040 ,China Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China Northeast Agricultural University, Harbin 150030, China)
出处
《食品工业科技》
CAS
CSCD
北大核心
2017年第21期151-156,161,共7页
Science and Technology of Food Industry
基金
大熊猫益生菌制剂的研究(SG1409)
黑龙江省应用技术研究与开发计划项目(GA15B203)
关键词
低聚异麦芽糖
橡子粉
液化
糖化
转苷
工艺
isomalto- oligosaccharide
acorn starch
liquefaction
saccharification
transglycosylation
technology
作者简介
张智（1964-），女，博士，教授，研究方向：微生物于发酵工程，E-mail：ldzhangzhi@163．com。
通讯作者：马建章（1937-），男，博士，院士，研究方向：野生动物生态与管理，E-mail：lq_233@163．com。
