摘要
[目的]对桑黄进行液体培养基的扩大培养,分析扩大培养过程中黄酮产量的变化。[方法]250、500、1 000 m L 3种锥形瓶分别装液体培养基150、300、600 m L,在28℃、180 r/min条件下培养。采用有机溶剂浸提法提取黄酮,分光光度法测定黄酮产量。[结果]在扩大培养过程中,桑黄胞内和胞外黄酮产量均呈现先下降再上升的趋势。[结论]该研究结果对桑黄胞内和胞外黄酮的生产、提取及应用具有现实的指导意义。
[Objective]The research aimed to expand the culture medium of Phellinus igniarius and analyze the change of flavonoid production during the culture process. [Method]150,300,600 m L liquid medium were stalled in 250,500,1 000 m L flasks respectively and incubated under 28 ℃,180 r/min conditions. Organic solvent extraction was used to extract flavonoids and spectrophotometry was used to measure flavonoids production. [Result]In the process of expanding the culture,the intracellular and extracellular flavonoids production of Phellinus igniarius showed a tendency to decrease first and then rise. [Conclusion]The results of this study have guiding significance for the production,extraction and application of intracellular and extracellular flavonoids from Phellinus igniarius.
出处
《安徽农业科学》
CAS
2017年第29期109-111,共3页
Journal of Anhui Agricultural Sciences
基金
山东省重点研发项目(2016GNC113019)
菏泽市科技发展计划项目(2012N002)
关键词
桑黄
液体培养基
扩大培养
黄酮产量
Phellinus igniarius
Liquid culture medium
Expanded culture
Flavonoid production
作者简介
杜睿绮（2000-）,女,山东菏泽人,高中生
通讯作者,副教授,硕士,从事食药用菌开发应用、微生物遗传育种研究.
