摘要
黄酮是药用真菌桑黄的主要活性成分之一。研究桑黄液体发酵培养基中不同碳源、氮源、无机盐对桑黄菌丝体生物量及胞内总黄酮产量的影响,筛选出最佳碳源、氮源、无机盐种类,并在单因素试验的基础上利用Box-Benhnken设计和响应面分析法优化其用量,得到有利于桑黄产胞内黄酮的液体发酵培养基最佳配方为:葡萄糖29.6 g/L,黄豆粉10.2 g/L,硫酸镁(MgSO4·7H2O)2.3 g/L,酵母膏1.1 g/L,KH2PO41.0 g/L。使用该优化液体发酵培养基培养桑黄,其胞内总黄酮产量可达186.75 mg/L。建立的桑黄菌丝体胞内总黄酮产量与培养基4种组分浓度的回归模型拟合性好,可用于预测胞内总黄酮产量。
Flavone is the main active ingredient of medicinal fungus Phellinus igniarius. In this study, the effect of different carbon sources, nitrogen sources, and inorganic salts in liquid fermentation medium on mycelium biomass and intra- cellular flavone yield of P. igniarius was investigated to determine the optimal carbon source, nitrogen source and inor- ganic salt. Based on single-factor tests, Box-Benhnken design combined with response surface analysis was conducted to optimize the dosage of carbon source, nitrogen source and inorganic salt. The optimized medium formulation of liquid fermentation medium for intracellular flavone was as follows, glucose 29.6 g/L, soybean flour 10.2 g/L, MgSQ4.7H2Q 2.3 g/L, yeast extract 1.1 g/L, and KH2PQ4 1.0 g/L. The yield of intracellular flavone of P. igniarius cultured in the optimized medium reached 186.75 mg/L. The established regression models between the yield of intracellular flavone and the concentration of 4 ingredients in the medium had good fitting performance, and could be used to predict the yield of intracellular flavone from P. igniarius.
出处
《蚕业科学》
CAS
CSCD
北大核心
2013年第6期1160-1165,共6页
ACTA SERICOLOGICA SINICA
基金
现代农业产业技术体系专项(No.CARS-22)
广东省自然科学基金博士启动项目(No.粤科基字[2010]3号)
广东省农业科学院院长基金项目(No.201006)
关键词
桑黄
液体发酵
培养基配方
黄酮
响应面分析法
Phellinus igniarius
Liquid fermentation
Medium formulation
Flavone
Response surface methodology
作者简介
第一作者信息：刘凡（1983-），男，博士，助理研究员。E—mail：liufan1234@126．com
通信作者信息：廖森泰，研究员，硕士生导师。E—mail：liaost@163．com Corresponding author．E-mail：liaost@163．com
