摘要
目的为了探究不同培养条件对桑黄菌丝体生长的影响,在短期内培养出质优、含多糖量高的菌丝体。方法通过观察、对比接种于不同的氮源、碳源培养基中桑黄菌丝体的生长曲线、生长形态、生物量、生理生化等指标,筛选出最适的桑黄菌丝体生长培养基。结果桑黄生长的最佳液体、固体培养基均为黄豆+玉米的综合培养基:黄豆50 g/L,玉米粒50 g/L,葡萄糖30 g/L,KH_2PO_4 3 g/L,MgSO_4 0.75g/L,维生素B_1 1 mg/L。结论最佳单因子结合对桑黄的生物量、多糖积累有协同效应,组合后的发酵体系中生物量达到32 g/L,多糖量达到5.661 mg/ml。
Objective To explore the influence of different culture conditions on the growth of Phellinus igniarius and to cultivate high-quality mycelia with high content of polysaccharose in the short run. Method Through the observation and comparison over the growth curve and morphology, biomass, and physiological and biochemical indicators of mycelia of Phellinus igniarius in different nitrogen source and carbon source media, the optimum culture medium for the growth of mycelia of Phellinus igniarius was singled out. Results The optimum liquid medium and solid medium for the growth of Phellinus igniarius are both the synthetic medium of corn and soybean, including soybean 50 g/L, niblet 50 g/L, glucose 30 g/L, KH_2PO_4 3 g/L, MgSO_4 0.75 g/L, and Vitamin B1 lmg/L. Conclusion The optimum single factor integration has synergistic effect on the accumulation of biomass and polysaccharose, and in the combined fermentation system, the biomass reached up to 32 g/L and polysaccharose reached up to 5.661 mg/ml.
出处
《现代中药研究与实践》
CAS
2017年第2期1-4,8,共5页
Research and Practice on Chinese Medicines
基金
国家自然科学基金(VZ8811137418)
关键词
桑黄菌丝体
多糖
黄酮
不同培养基
Phellinus igniarius
polysaccharide
flavonoid
different media
作者简介
周蔓（1988-），女，在读硕士研究生，研究方向：天然药物的研究与开发。E-mail：787669175@qq．com
通讯作者：李萍（1962-），女，博士研究生，教授，研究方向：生物资源与天然药物的研究开发。E-mail：wuping4535@sina．com
