摘要
【目的】为探明基质对豹皮樟春梢扦插生根能力的影响及规模化扦插育苗基质应用提供依据。【方法】以成龄豹皮樟春梢为繁殖材料,用生长调节剂GGR 50 mg/L处理后,分别扦插在老鹰茶原生地紫色沙壤土(下称紫色土)、珍珠岩、蛭石3种基质上,研究不同基质对插穗的扦插生根率、愈伤组织诱导期的营养物质含量、关联酶活性以及内源激素水平的影响,并进行多重比较。【结果】紫色土基质插穗生根率最高,为70.64%,与蛭石(55.86%)、珍珠岩(62.29%)基质间极显著(P<0.01);插穗中总酚含量与PPO酶活性呈正相关,Chl含量与SOD酶活性、CAT酶呈正相关,光合作用能力增强导致插穗叶片中分解产生的活性氧、丙二醛、脯氨酸等代谢废物含量增加;内源IAA水平与生根率呈负相关。【结论】基质p H、交换性盐基含量等理化性状的差异导致内含物质含量、相关酶活性等产生,插条中生根抑制物质、光毒性和内源IAA是导致不同基质插穗生根能力产生差异原因。
[ Objective ] This study aimed to verify the effect of different substrates on rooting capability of L. coreana shoot cuttings and provide the basis for substrate application of large-scale cutting seedling-raising. [ Method ] The effects of different substrates on rooting rate of cuttings and nutrient content, relative enzymatic activity and endogenous hormone level of calli at induction stage are studied by culturing a- dult L. coreana spring shoots treated with 50 mg/L GGR in three different substrates of purple soil, perlite and vermiculite respectively. [ Result]The rooting rates of the cuttings cultured in purple soil, perlite and vermiculite were 70.64 % , 62.29 % and 55.86 % , respec- tively, and there was a difference in rooting rate very significantly among three substrates. Total phenols content of cuttings was positively re- lated to PPO activity. The Chl content was positively related to SOD and CAT activity. The enhancement of photosynthetic ability brought a- bout increase of reactive oxygen, MDA and proline content in cuttings＇ leaves. The endogenesis IAA level was negatively related to rooting rate of cuttings. [ Conclusion ] The difference in pH and exchangeable bases content among three substrates leads to the difference in internal substance content and relative enzymatic activity of cuttings＇ leaves. The rooting inhibitors, phototoxicity and endogenesis IAA in the cuttings are main factors to bring about the difference in cuttings＇ rooting ability among three substrates.
作者
屈坤杰
王济红
祁翔
周少奇
QU Kun-jie WANG Ji-hong QI Xiang ZHOU Shao-qi(Guizhou University, Guizhou Guiyang 550025, China Guizhou Biologieal Institute, Guizhou Guiyang 550009, China Guizhou Academy of Seienees, Guizhou Guiyang 550001, China)
出处
《西南农业学报》
CSCD
北大核心
2017年第7期1522-1527,共6页
Southwest China Journal of Agricultural Sciences
基金
贵州省科技厅产业化项目"老鹰茶产业化技术产学研示范基地建设"
关键词
豹皮樟
生根能力
内因
基质种类
Litsea careana
Rooting capability
Endogenesis
Substrate type
作者简介
屈坤杰（1989-），男，湖北汉川人，在读硕士，研究方向：环境生态学，E-mail：502971460@qq．com
为通讯作者：周少奇，E-mail：2975742087@qq．com。
