摘要
白鸡冠茶树是光照敏感型茶树,为挖掘与白鸡冠茶树在光照和遮阴条件下叶色变化相关的关键基因,选取遮阴和恢复光照处理白鸡冠第二叶5个时期的转录组数据,通过加权网络共表达分析(WGCNA)方法构建模块.6580个差异表达基因构建的WGCNA网络被分为15个模块,其中darkseagreen模块与叶绿素含量相对值(SPAD值)显著正相关,honeydew模块和SPAD值显著负相关.GO富集结果表明darkseagreen模块显著富集到初级代谢过程的调节和压力响应等过程,而honeydew模块在叶绿体和单器官代谢等过程显著富集.从darkseagreen模块和honeydew模块中发掘到数个潜在核心基因,并采用Cytoscape对darkseagreen模块和honeydew模块的基因绘制互作网络图,进而从darkseagreen模块筛选出2个基因(CSS0019351、CSS0045917)和2个转录因子(CSS0046793和CSS0018417),在honeydew模块筛选出2个基因(CSS0031492和CSS0036305)和1个转录因子(CSS0023211).本研究通过WGCNA方法筛选出2个具有生物学意义的模块,进一步挖掘得到的7个关键基因和转录因子与叶绿素合成和叶绿体发育密切相关.
Baijiguan(Camellia sinensis(L.)O.Ktunze cv.Baijiguan),is a light-sensitive tea cultivar.To explore key genes associated with changes in leaf color of the Baijiguan tea cultivar under light and shade conditions,transcriptome data from five periods of shade and restored light treatment of the second leaf of Baijiguan were selected to construct modules using the WGCNA method.The WGCNA network constructed from 6580 differentially expressed genes was divided into 15 modules,which were significantly positively correlated with the relative chlorophyll content(SPAD value),and the honeydew module and SPAD value were significantly negatively correlated.GO enrichment analysis revealed that in the darkseagreen module,the genes were significantly enriched in processes such as the regulation of primary metabolic pathways and stress responses,and the honeydew module genes were significantly enriched in processes such as chloroplast and single-organism metabolic pathways.Several potential core genes were identified from the darkseagreen and honeydew modules,and Cytoscape was employed to map the interaction network of genes in these two modules.Two genes(CSS0019351 and CSS0045917)and two transcription factors(CSS0046793 and CSS0018417)were screened from the darkseagreen module,and the honeydew module also had two genes(CSS0031492 and CSS0036305)and one transcription factor(CSS0023211)that were selected.In summary,the WGCNA method identified two biologically meaningful modules.Seven key hub genes and transcription factors were further mined and found to be closely related to chlorophyll synthesis and chloroplast development.
作者
王涛
王艺清
周喆
赵泽昊
曹士先
陈志丹
孙威江
WANG Tao;WANG Yiqing;ZHOU Zhe;ZHAO Zehao;CAO Shixian;CHEN Zhidan;SUN Weijiang(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Anxi College of Tea Science,Fujian Agriculture and Forestry University,Quanzhou 362400,China;Engineering Technology and Research Center of Fujian Tea Industry,Fuzhou 350002,China;Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Wuyi Star Tea Industrial Company Limited,Nanping 354300,China)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2023年第2期377-385,共9页
Chinese Journal of Applied and Environmental Biology
基金
福建省高校产学合作项目(2019N5007)
国家自然科学基金项目(31770732)
福建农林大学茶产业链科技创新与服务体系建设项目(K1520005A04)
福建张天福茶叶发展基金会科技创新基金项目(FJZTF01)资助
作者简介
通信作者:陈志丹,E-mail:asbulletdan@163.com;通信作者:孙威江,E-mail:swj8103@126.com
