摘要
以红树莓加工过程中产生的副产物红树莓籽为试验条件,在单因素试验基础上,采用Box-Behnken试验设计方法优化超高压辅助提取红树莓籽黄酮的工艺条件,结果表明:优化工艺条件为乙醇体积分数70%、超高压压力300 MPa、料液比1∶30、保压时间5 min。在此条件下红树莓籽中黄酮得率为55.4 mg/g,与预测值55.7mg/g相吻合。抗氧化活性研究表明:在一定浓度范围,树莓籽黄酮具有较高的抗氧化能力,对O_2^-·自由基的IC50为0.24 mg/m L。
Red raspberry pomace was selected as the raw material.On the basis of single factors experiments,the Box-Behnken was used to study the extraction efficiency of flavonoids with the aid of ultra high pressure energy.The results show that the regression equation fit well with experimental data,the optimum conditions of extraction were：ethanol concentration 70%,ultra pressure 300 MPa,liquid-solid ratio 1 ∶ 30,extraction time 5 min.Under the optimal conditions,the experimental yield 38.6 mg/g was close to the predicted 55.7 mg/g.Results indicated that the flavonoids from raspberry seeds had an obvious antioxidant activity,the IC50 values of O2-· free radical scavenging abilities were 0.24 mg/mL.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2017年第2期101-109,共9页
Journal of Chinese Institute Of Food Science and Technology
基金
"十二五"国家科技支撑计划项目(2012BAD29B06)
辽宁省科技厅农业攻关计划项目(2011205001)
辽宁省食品安全重点实验室暨辽宁省高校重大科技平台开放课题项目(LNSAKF2011028)
关键词
红树莓籽
黄酮
超高压提取
响应面
抗氧化活性
raspberry seeds
flavonoids
ultra high pressure assisted extraction
the response surface
antioxidant activity
作者简介
李萌萌，女，1989年出生，硕士生
通讯作者：吕长鑫E-mail：lvchangxin6666@163．com
